Sam:LabNotes/Microbione/2009-2-5: Difference between revisions

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>Sam Chiang
>Sam Chiang
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==Results==
==Results==


[[Image:sam020409.jpg|frame|100px|'''FIG.1 Realtime amplification plot (no log in flourescent)''']]
[[Image:sam020409.jpg|frame|50px|'''FIG.1 Realtime amplification plot (no log in flourescent)''']]




[[Image:sam020409-log.jpg|frame|100px|'''FIG.2 Realtime amplification plot (with log in flourescent)''']]
[[Image:sam020409-log.jpg|frame|50px|'''FIG.2 Realtime amplification plot (with log in flourescent)''']]


==Discussion==
==Discussion==

Revision as of 20:54, 5 February 2009

Follow up: Testing human 18S and Bac (E. coli) 16S primers using realtime PCR

Exp. Design

Template         gDNA      H2O
                --------  ---
Primer: 18S-211  #1   #2   #3    (#1, #2 - replicates)
        18S-306  #4   #5   #6    (#4, #5 - replicates)


Results

File:Sam020409.jpg
FIG.1 Realtime amplification plot (no log in flourescent)


File:Sam020409-log.jpg
FIG.2 Realtime amplification plot (with log in flourescent)

Discussion

  1. The amplification curves are not distinguishable (Sample vs. Blank) within 20 clycles.
    1. The amplifications cycless were not enough?
    2. The amplifications are not working?
      1. Primers' specificity? binding affinity?
      2. Concentration of templates?

Suggestion