Kun:LabNotes/ExonomeSeq: Difference between revisions

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Revision as of 23:46, 1 April 2008

2007 <calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2007/11/01 view=threemonths </calendar> 2008 <calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2008/02/01 view=threemonths </calendar>

<calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2008/05/01 view=threemonths </calendar>

Probe Design

  • The first probe set: Kun:LabNotes/ExonomeSeq/2007-11-15
  • One 55k library received on 12/12/2007, OLID: 018274110001, 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.
  • The second probe set: Kun:LabNotes/ExonomeSeq/2007-12-21
  • Two Agilent 55k libraries were ordered on 01/04/2008.
  1. Cosmic3760 0189191
  2. CpgCes: Cpg28k + CES27k 0189181

Received 01/24/2008. 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.

Overview of experiments

Sequencing library construction

Random primed

  • Forward primer (SolFN6):CGACGCTCTTCCGATCTNNNNNN Tm left = 59.20C
  • Reverse primer (SolRN6):CATACGAGCTCTTCCGATCTNNNNNN Tm left = 59.45C

USER-Lig