Kun:LabNotes/ExonomeSeq: Difference between revisions
Jump to navigation
Jump to search
mNo edit summary |
mNo edit summary |
||
Line 1: | Line 1: | ||
2008 | 2008 | ||
<calendar> | <calendar> | ||
Line 11: | Line 4: | ||
format=%name/%year-%month-%day | format=%name/%year-%month-%day | ||
date=2008/02/01 | date=2008/02/01 | ||
view= | view=oneyear | ||
</calendar> | </calendar> | ||
2007 | |||
<calendar> | <calendar> | ||
name=Kun:LabNotes/ExonomeSeq | name=Kun:LabNotes/ExonomeSeq | ||
format=%name/%year-%month-%day | format=%name/%year-%month-%day | ||
date= | date=2007/11/01 | ||
view=threemonths | view=threemonths | ||
</calendar> | </calendar> | ||
==Probe Design== | ==Probe Design== | ||
*The first probe set: [[Kun:LabNotes/ExonomeSeq/2007-11-15]] | *The first probe set: [[Kun:LabNotes/ExonomeSeq/2007-11-15]] |
Revision as of 22:57, 5 April 2008
2008 <calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2008/02/01 view=oneyear </calendar>
2007 <calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2007/11/01 view=threemonths </calendar>
Probe Design
- The first probe set: Kun:LabNotes/ExonomeSeq/2007-11-15
- One 55k library received on 12/12/2007, OLID: 018274110001, 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.
- The second probe set: Kun:LabNotes/ExonomeSeq/2007-12-21
- Two Agilent 55k libraries were ordered on 01/04/2008.
- Cosmic3760 0189191
- CpgCes: Cpg28k + CES27k 0189181
Received 01/24/2008. 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.
Overview of experiments
Sequencing library construction
Random primed
- Forward primer (SolFN6):CGACGCTCTTCCGATCTNNNNNN Tm left = 59.20C
- Reverse primer (SolRN6):CATACGAGCTCTTCCGATCTNNNNNN Tm left = 59.45C