Daniel:Notebook/HiResChrPaint/2014-8-23: Difference between revisions
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>Djacobse (Created page with "=Padlock Probes (Started 8/13/2014)= Back to Calendar ==Hybridization ([[Daniel:Notebook/HiRes...") |
>Djacobse No edit summary |
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[[ Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[ Daniel:Notebook/HiResChrPaint|Back to Calendar]] | ||
==Hybridization ([[Daniel:Notebook/HiResChrPaint/2014-8-21|From 8/21/2014]]) | ==Hybridization ([[Daniel:Notebook/HiResChrPaint/2014-8-21|From 8/21/2014]])== | ||
#Remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes | |||
#Incubate in 2X SSCT at room temperature for 10 minutes | |||
#Incubate in 0.2X SSC at room temperature for 10 minutes | |||
#Dry with kimwipes; do not let the surface containing the cells touch the kimwipes | |||
#Add 20 uL DAPI + Slow Fade Gold to center of dish | |||
#Cover with 22x22 #1.5 coverslip; seal with nail polish | |||
##Allow at least 30 minutes for nail polish to dry | |||
##Slides are quite stable for several months at 4C |
Revision as of 18:29, 23 August 2014
Padlock Probes (Started 8/13/2014)
Hybridization (From 8/21/2014)
- Remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes
- Incubate in 2X SSCT at room temperature for 10 minutes
- Incubate in 0.2X SSC at room temperature for 10 minutes
- Dry with kimwipes; do not let the surface containing the cells touch the kimwipes
- Add 20 uL DAPI + Slow Fade Gold to center of dish
- Cover with 22x22 #1.5 coverslip; seal with nail polish
- Allow at least 30 minutes for nail polish to dry
- Slides are quite stable for several months at 4C