Daniel:Notebook/RNAFISH/2014-8-30: Difference between revisions
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=Trial Run ([[Daniel: | =Trial Run ([[Daniel:Notebook/RNAFISH/2014-8-29|Started 08/29/2014]])= | ||
[[Daniel:Notebook/RNAFISH|Back to Calendar]] | [[Daniel:Notebook/RNAFISH|Back to Calendar]] |
Revision as of 18:31, 30 August 2014
Trial Run (Started 08/29/2014)
Tissue Section Preparation (From 08/29/2014)
- Wash the tissue in PBS, 3 × 30 min
- Incubate the tissue in a sucrose series with increasing concentrations:
- 10% for 1 h
- 20% for 1 h
- 30% at 4C overnight