Daniel:Notebook/RNAFISH/2014-8-30: Difference between revisions

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##20% for 1 h  
##20% for 1 h  
##30% at 4C overnight
##30% at 4C overnight
==Oligo Set Preparations==
These are the oligos that I ordered on

Revision as of 23:04, 30 August 2014

Trial Run (Started 08/29/2014)

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Tissue Section Preparation (From 08/29/2014)

  1. Wash the tissue in PBS, 3 × 30 min
  2. Incubate the tissue in a sucrose series with increasing concentrations:
    1. 10% for 1 h
    2. 20% for 1 h
    3. 30% at 4C overnight

Oligo Set Preparations

These are the oligos that I ordered on