Daniel:Notebook/RNAFISH/2014-8-30: Difference between revisions
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##20% for 1 h | ##20% for 1 h | ||
##30% at 4C overnight | ##30% at 4C overnight | ||
==Oligo Set Preparations== | |||
These are the oligos that I ordered on |
Revision as of 23:04, 30 August 2014
Trial Run (Started 08/29/2014)
Tissue Section Preparation (From 08/29/2014)
- Wash the tissue in PBS, 3 × 30 min
- Incubate the tissue in a sucrose series with increasing concentrations:
- 10% for 1 h
- 20% for 1 h
- 30% at 4C overnight
Oligo Set Preparations
These are the oligos that I ordered on