AlanFung:LabNotes/2014/2014-9-5: Difference between revisions

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[[File:09052014_workflow.jpg|200px]]
[[File:09052014_workflow.jpg|200px]]
===End Repair Reaction Setup===
===End Repair Reaction Setup===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
|- style="background-color:#BFBFBF" align="center"
| width="151" height="28" | Component
| width="65" | 1 Library
"| width=""113"" | 15 Libraries
(+5% excess)"
|-  align="center"
| height="14" | End Repair Master Mix:
| align="center" | &nbsp;
| align="center" | &nbsp;
|-  align="center"
| height="14" | Water
| align="center" | 8
| align="center" | 126
|-  align="center"
| height="14" | 10X Kapa End Repair Buffer
| align="center" | 7
| align="center" | 110.25
|-  align="center"
| height="14" | Kapa End Repair Enzyme Mix
| align="center" | 5
| align="center" | 78.75
|- style="font-weight:bold" align="center"
| height="14" | Total master mix volume
| align="center" | 20
| align="center" | 315
|}
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
|-  align="center"
|-  align="center"
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*Mix, and incubate at 20C for 30mins
*Mix, and incubate at 20C for 30mins
*Proceed immediately to the next step
*Proceed immediately to the next step
===End Repair Cleanup===
===End Repair Cleanup===

Revision as of 21:58, 4 September 2014

KAPA Library Preparation Kit illumina Platforms

Parts

  • Kapa Library Preparation Kit illumina platforms KR0935 V1.13

Workflow

File:09052014 workflow.jpg

End Repair Reaction Setup

Component 1 Library

"| width=""113"" | 15 Libraries (+5% excess)"

End Repair Master Mix:    
Water 8 126
10X Kapa End Repair Buffer 7 110.25
Kapa End Repair Enzyme Mix 5 78.75
Total master mix volume 20 315
Fragmented, double-stranded DNA 50ul
End Repair Master Mix 20ul
Total rection Mix 70ul
  • Mix, and incubate at 20C for 30mins
  • Proceed immediately to the next step

End Repair Cleanup