AlanFung:LabNotes/2014/2014-9-5: Difference between revisions
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[[File:09052014_workflow.jpg|200px]] | [[File:09052014_workflow.jpg|200px]] | ||
===End Repair Reaction Setup=== | ===End Repair Reaction Setup=== | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext> | |||
|- style="background-color:#BFBFBF" align="center" | |||
| width="151" height="28" | Component | |||
| width="65" | 1 Library | |||
"| width=""113"" | 15 Libraries | |||
(+5% excess)" | |||
|- align="center" | |||
| height="14" | End Repair Master Mix: | |||
| align="center" | | |||
| align="center" | | |||
|- align="center" | |||
| height="14" | Water | |||
| align="center" | 8 | |||
| align="center" | 126 | |||
|- align="center" | |||
| height="14" | 10X Kapa End Repair Buffer | |||
| align="center" | 7 | |||
| align="center" | 110.25 | |||
|- align="center" | |||
| height="14" | Kapa End Repair Enzyme Mix | |||
| align="center" | 5 | |||
| align="center" | 78.75 | |||
|- style="font-weight:bold" align="center" | |||
| height="14" | Total master mix volume | |||
| align="center" | 20 | |||
| align="center" | 315 | |||
|} | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext> | ||
|- align="center" | |- align="center" | ||
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*Mix, and incubate at 20C for 30mins | *Mix, and incubate at 20C for 30mins | ||
*Proceed immediately to the next step | *Proceed immediately to the next step | ||
===End Repair Cleanup=== | ===End Repair Cleanup=== |
Revision as of 21:58, 4 September 2014
KAPA Library Preparation Kit illumina Platforms
Parts
- Kapa Library Preparation Kit illumina platforms KR0935 V1.13
Workflow
End Repair Reaction Setup
Component | 1 Library
"| width=""113"" | 15 Libraries (+5% excess)" | |
End Repair Master Mix: | ||
Water | 8 | 126 |
10X Kapa End Repair Buffer | 7 | 110.25 |
Kapa End Repair Enzyme Mix | 5 | 78.75 |
Total master mix volume | 20 | 315 |
Fragmented, double-stranded DNA | 50ul |
End Repair Master Mix | 20ul |
Total rection Mix | 70ul |
- Mix, and incubate at 20C for 30mins
- Proceed immediately to the next step