AlanFung:LabNotes/2014/2014-9-8: Difference between revisions
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==PAGE Gel Verification== | ==PAGE Gel Verification== | ||
[[File:ZhangLab_2 2014-09-08 16hr 52min.jpg]] | [[File:ZhangLab_2 2014-09-08 16hr 52min.jpg]] | ||
*Bands ~110bp is most likely primer dimers, it was not removed by the beads as 1.7X beads will only remove anything below 100bp |
Revision as of 00:58, 9 September 2014
qPCR Test to verifiy End repair, A-tailing & Adapter Ligation
Component | 1 Library | 19 Libraries + 3% |
qPCR Master Mix: | ||
Adapter ligated DNA | 1 | 19.6 |
10uM PCR_F | 0.5 | 9.8 |
10uM PCR_R.Ind2 | 0.5 | 9.8 |
2X Kapa Fast | 12.5 | 244.6 |
Water | 10.5 | 205.5 |
Total master mix volume | 25 | 489.3 |
- 98C 2min --> (98C 15sec -> 64C 20sec -> 72C 30sec) x 12 cycles --> 72C 3min --> 15C Hold
PAGE Gel Verification
File:ZhangLab 2 2014-09-08 16hr 52min.jpg
- Bands ~110bp is most likely primer dimers, it was not removed by the beads as 1.7X beads will only remove anything below 100bp