Arichard:Notebook/midas140911: Difference between revisions

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>Andrew
(Created page with "==MIDAS, September 11, 2014 (midas140911)== * Gwen asked for a run with only cerebellum, all of which is NeuN +, with both AD patient 25-00 and control patient 7-03 in the sa...")
 
>Andrew
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===Size select gels after Nextera===
===Size select gels after Nextera===
[[File:midas140911GelsSlide1.jpg|400px]]
[[File:midas140911GelsSlide2.jpg|400px]]
[[File:midas140911GelsSlide3.jpg|400px]]
[[File:midas140911GelsSlide4.jpg|400px]]
[[File:midas140911GelsSlide5.jpg|400px]]
[[File:midas140911GelsSlide6.jpg|400px]]

Revision as of 22:01, 19 September 2014

MIDAS, September 11, 2014 (midas140911)

  • Gwen asked for a run with only cerebellum, all of which is NeuN +, with both AD patient 25-00 and control patient 7-03 in the same run.
  • Based on positive results from the last run (midas140828), I removed the buffer headspace from all microwell arrays before adding oil overlay. This was an effort to prevent runaway reactions.
  • Also based on positive results from the last run, I skipped ALS/NS/PolI and EtOH precipitation before Nextera. This made the run much faster.

Samples

Size select gels after Nextera

File:Midas140911GelsSlide1.jpg

File:Midas140911GelsSlide2.jpg

File:Midas140911GelsSlide3.jpg

File:Midas140911GelsSlide4.jpg

File:Midas140911GelsSlide5.jpg

File:Midas140911GelsSlide6.jpg