Arichard:Notebook/midas140911: Difference between revisions
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>Andrew (Created page with "==MIDAS, September 11, 2014 (midas140911)== * Gwen asked for a run with only cerebellum, all of which is NeuN +, with both AD patient 25-00 and control patient 7-03 in the sa...") |
>Andrew |
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===Size select gels after Nextera=== | ===Size select gels after Nextera=== | ||
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Revision as of 22:01, 19 September 2014
MIDAS, September 11, 2014 (midas140911)
- Gwen asked for a run with only cerebellum, all of which is NeuN +, with both AD patient 25-00 and control patient 7-03 in the same run.
- Based on positive results from the last run (midas140828), I removed the buffer headspace from all microwell arrays before adding oil overlay. This was an effort to prevent runaway reactions.
- Also based on positive results from the last run, I skipped ALS/NS/PolI and EtOH precipitation before Nextera. This made the run much faster.
Samples
Size select gels after Nextera
File:Midas140911GelsSlide1.jpg
File:Midas140911GelsSlide2.jpg
File:Midas140911GelsSlide3.jpg
File:Midas140911GelsSlide4.jpg