Arichard:Notebook/midas140917: Difference between revisions

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===Gels===
===Gels===
[[File:midas140917GelsSlide1.jpg|400px]]
[[File:midas140917GelsSlide2.jpg|400px]]
[[File:midas140917GelsSlide3.jpg|400px]]
[[File:midas140917GelsSlide4.jpg|400px]]
[[File:midas140917GelsSlide5.jpg|400px]]
[[File:midas140917GelsSlide6.jpg|400px]]


===Sequencing===
===Sequencing===

Revision as of 17:09, 24 September 2014

MIDAS run from September 17, 2014

Sample tracking

Sample # Sample description Array Image pos. Index # Barcode
1 NeuN+ Cbl 7-03 sorted May 20 1 1 1 ATCACG
2 NeuN+ Cbl 7-03 sorted May 21 1 2 2 CGATGT
3 NeuN+ Cbl 7-03 sorted May 22 2 3 4 TGACCA
4 NeuN+ Cbl 7-03 sorted May 23 2 5 5 ACAGTG
5 NeuN+ Cbl 7-03 sorted May 24 3 6 6 GCCAAT
6 NeuN+ Cbl 7-03 sorted May 25 3 7 7 CAGATC
7 NeuN+ Cbl 7-03 sorted May 26 3 7 10 TAGCTT
8 NeuN+ Cbl 7-03 sorted May 27 4 8 8 ACTTGA
9 NTC from loaded array 4 8 11 GGCTAC
10 NeuN+ Cbl 25-00 sorted May 27 5 10 12 CTTGTA
11 NeuN+ Cbl 25-00 sorted May 28 5 10 13 CCATGA
12 NeuN+ Cbl 25-00 sorted May 29 5 11 14 AAGACA
13 NeuN+ Cbl 25-00 sorted May 30 5 11 15 CTTCCT
14 NeuN+ Cbl 25-00 sorted May 31 6 12 25 TACTGC
15 NeuN+ Cbl 25-00 sorted May 32 6 12 26 TCGTGA
16 NeuN+ Cbl 25-00 sorted May 33 6 13 16 GGGGTT
17 NeuN+ Cbl 25-00 sorted May 34 6 13 27 TACGCG
18 NeuN+ Cbl 25-00 sorted May 35 7 14 28 AGGTGC
19 NeuN+ Cbl 25-00 sorted May 36 7 14 29 ACGAAC
20 NeuN+ Cbl 25-00 sorted May 37 7 15 30 TTAGTG
21 NeuN+ Cbl 25-00 sorted May 38 8 16 31 CACCAC
22 NeuN+ Cbl 25-00 sorted May 39 8 16 33 CGCAAC
23 NeuN+ Cbl 25-00 sorted May 40 8 17 34 AACTGA
24 NTC from loaded array 8 17 35 ATCGGG
  • I used a different type of array, the last one from the Groisman lab.
  • It contained a type of bulk blocking treatment that would wet and block PDMS pores without BSA.
  • They told me to use it without BSA, but I used BSA anyways.
  • I saw a lot of wrinkling in the bottom of the array due to its thinness. This created a lot of issues with imaging and picking.

Gels

File:Midas140917GelsSlide1.jpg File:Midas140917GelsSlide2.jpg File:Midas140917GelsSlide3.jpg File:Midas140917GelsSlide4.jpg File:Midas140917GelsSlide5.jpg File:Midas140917GelsSlide6.jpg

Sequencing