Blue:RNA-Seq Experiments:09232014: Difference between revisions

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*20140916 (SSCs) ( [https://docs.google.com/spreadsheet/ccc?key=0AmTJ0NCGGrdpdGtiYTFWQ0RiTnJQY0FmZnRoY3VYOEE&usp=drive_web#gid=59 c1_expr_sop_non-hNuc Shared google doc] )
*20140916 (SSCs) ( [https://docs.google.com/spreadsheet/ccc?key=0AmTJ0NCGGrdpdGtiYTFWQ0RiTnJQY0FmZnRoY3VYOEE&usp=drive_web#gid=59 c1_expr_sop_non-hNuc Shared google doc] )


== MiSeq Test ==


*Pooled 4ul of each sample (1x0nuc 7x1nuc) for MiSeq Test for each exp listed above
*Pooled 4ul of each sample (1x0nuc 7x1nuc) for MiSeq Test for each exp listed above
Line 13: Line 14:
[[File:09232014_PCR2_Gel1.jpg]]
[[File:09232014_PCR2_Gel1.jpg]]


Samples:
 
Individual Samples:
 
 
{| {{table}} border
| align="center" style="background:#f0f0f0;"|'''Library'''
| align="center" style="background:#f0f0f0;"|'''Index'''
|-
| 20140909A_0C03||S501/N701
|-
| 20140909A_1C14||S501/N702
|-
| 20140909A_1C27||S501/N703
|-
| 20140909A_1C62||S501/N704
|-
| 20140909A_1C09||S502/N701
|-
| 20140909A_1C20||S502/N702
|-
| 20140909A_1C33||S502/N703
|-
| 20140909A_1C68||S502/N704
|-
| 20140918_0C15||S501/N708
|-
| 20140918_1C25||S502/N708
|-
| 20140918_1C19||S501/N709
|-
| 20140918_1C27||S502/N709
|-
| 20140918_1C20||S506/N708
|-
| 20140918_1C38||S507/N708
|-
| 20140918_1C55||S506/N709
|-
| 20140918_1C67||S507/N709
|-
| 20140916_0C39||S504/N703
|-
| 20140916_1C74||S504/N704
|-
| 20140916_1C18||S504/N705
|-
| 20140916_1C29||S504/N706
|-
| 20140916_1C45||S505/N703
|-
| 20140916_1C80||S505/N704
|-
| 20140916_1C24||S505/N705
|-
| 20140916_1C35||S505/N706
|-
|}
 
== Oct1 HiSeq Library: SSCs ==
 
 
*Combined 1ul from each nextera library
*Beads purified 0.8x twice
*Re-suspended in 10ul
*Ran 1ul on gel:
 
[[File:10012014_PCR2_Gel1.jpg]]

Latest revision as of 00:51, 2 October 2014

C1 Runs[edit]

MiSeq Test[edit]

  • Pooled 4ul of each sample (1x0nuc 7x1nuc) for MiSeq Test for each exp listed above
  • Beads purified twice (0.8x)
  • Ran 1ul of 10ul onto gel:

File:09232014 PCR2 Gel1.jpg


Individual Samples:


Library Index
20140909A_0C03 S501/N701
20140909A_1C14 S501/N702
20140909A_1C27 S501/N703
20140909A_1C62 S501/N704
20140909A_1C09 S502/N701
20140909A_1C20 S502/N702
20140909A_1C33 S502/N703
20140909A_1C68 S502/N704
20140918_0C15 S501/N708
20140918_1C25 S502/N708
20140918_1C19 S501/N709
20140918_1C27 S502/N709
20140918_1C20 S506/N708
20140918_1C38 S507/N708
20140918_1C55 S506/N709
20140918_1C67 S507/N709
20140916_0C39 S504/N703
20140916_1C74 S504/N704
20140916_1C18 S504/N705
20140916_1C29 S504/N706
20140916_1C45 S505/N703
20140916_1C80 S505/N704
20140916_1C24 S505/N705
20140916_1C35 S505/N706

Oct1 HiSeq Library: SSCs[edit]

  • Combined 1ul from each nextera library
  • Beads purified 0.8x twice
  • Re-suspended in 10ul
  • Ran 1ul on gel:

File:10012014 PCR2 Gel1.jpg