Daniel:Notebook/HiResChrPaint/2014-9-22: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
(Created page with "=Slide FISH (Started 09/19/2014)= Back to Calendar ==Microscopy== ===Dystrophin=== <gallery h...")
 
>Djacobse
Line 13: Line 13:
File:20140922_DMD488_Cot1546.lif_Zoom1_ch00.jpg|Zoom 1
File:20140922_DMD488_Cot1546.lif_Zoom1_ch00.jpg|Zoom 1
</gallery>
</gallery>
These images don't tell me a great deal one way or another.  There appears to be good dystrophin signal, so I think that the probes must be working, but it is difficult to check whether or not that signal is dystrophin itself.  The Cot1 continues to perform admirably.


===Chr21 Padlock Probes===
===Chr21 Padlock Probes===
<gallery heights=500px widths=500px perrow=3 caption="PPRL-488 (green) and Cot1-546 (red)">
File:20140922_PPRL488_Cot1546.lif_Field1_NoThreshold_ch00.jpg|No Threshold
File:20140922_PPRL488_Cot1546.lif_Field1_NormalThreshold_ch00.jpg|Normal Zero Threshold (20/255)
File:20140922_PPRL488_Cot1546.lif_Field1_BestThreshold_ch00.jpg|Best Threshold (100/255)
</gallery>
These images have much stronger green signal, corresponding to the Padlock Probes from Rui (PPRL).  The comforting thing is that since there are thousands of these probes, I would expect a stronger signal.  However, they are only supposed to paint chromosome 21, and even then not the whole thing.  Therefore I should only see 2 or so concentrated dots, but I typically see much stronger signal than that.  [[Daniel:Notebook/HiResChrPaint/2014-9-22|Tomorrow]]'s microscopy may provide more insight.
[[File:20140922_PPRL488_Cot1546.lif_TileScan_ch00.jpg|center|1000px|PPRL-488 (green) and Cot1-546 (red), tile scan]]


[[Category:HRCP]] [[Category:Microscopy]] [[Category:20140919]]
[[Category:HRCP]] [[Category:Microscopy]] [[Category:20140919]]

Revision as of 19:14, 24 September 2014

Slide FISH (Started 09/19/2014)

Back to Calendar

Microscopy

Dystrophin

These images don't tell me a great deal one way or another. There appears to be good dystrophin signal, so I think that the probes must be working, but it is difficult to check whether or not that signal is dystrophin itself. The Cot1 continues to perform admirably.

Chr21 Padlock Probes

These images have much stronger green signal, corresponding to the Padlock Probes from Rui (PPRL). The comforting thing is that since there are thousands of these probes, I would expect a stronger signal. However, they are only supposed to paint chromosome 21, and even then not the whole thing. Therefore I should only see 2 or so concentrated dots, but I typically see much stronger signal than that. Tomorrow's microscopy may provide more insight.