Daniel:Notebook/HiResChrPaint/2014-9-22: Difference between revisions
>Djacobse (Created page with "=Slide FISH (Started 09/19/2014)= Back to Calendar ==Microscopy== ===Dystrophin=== <gallery h...") |
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File:20140922_DMD488_Cot1546.lif_Zoom1_ch00.jpg|Zoom 1 | File:20140922_DMD488_Cot1546.lif_Zoom1_ch00.jpg|Zoom 1 | ||
</gallery> | </gallery> | ||
These images don't tell me a great deal one way or another. There appears to be good dystrophin signal, so I think that the probes must be working, but it is difficult to check whether or not that signal is dystrophin itself. The Cot1 continues to perform admirably. | |||
===Chr21 Padlock Probes=== | ===Chr21 Padlock Probes=== | ||
<gallery heights=500px widths=500px perrow=3 caption="PPRL-488 (green) and Cot1-546 (red)"> | |||
File:20140922_PPRL488_Cot1546.lif_Field1_NoThreshold_ch00.jpg|No Threshold | |||
File:20140922_PPRL488_Cot1546.lif_Field1_NormalThreshold_ch00.jpg|Normal Zero Threshold (20/255) | |||
File:20140922_PPRL488_Cot1546.lif_Field1_BestThreshold_ch00.jpg|Best Threshold (100/255) | |||
</gallery> | |||
These images have much stronger green signal, corresponding to the Padlock Probes from Rui (PPRL). The comforting thing is that since there are thousands of these probes, I would expect a stronger signal. However, they are only supposed to paint chromosome 21, and even then not the whole thing. Therefore I should only see 2 or so concentrated dots, but I typically see much stronger signal than that. [[Daniel:Notebook/HiResChrPaint/2014-9-22|Tomorrow]]'s microscopy may provide more insight. | |||
[[File:20140922_PPRL488_Cot1546.lif_TileScan_ch00.jpg|center|1000px|PPRL-488 (green) and Cot1-546 (red), tile scan]] | |||
[[Category:HRCP]] [[Category:Microscopy]] [[Category:20140919]] | [[Category:HRCP]] [[Category:Microscopy]] [[Category:20140919]] |
Revision as of 19:14, 24 September 2014
Slide FISH (Started 09/19/2014)
Microscopy
Dystrophin
- 20140922 DMD488 Cot1546.lif Field1 ch00.jpg
Field 1
- 20140922 DMD488 Cot1546.lif Field2 ch00.jpg
Field 2
- 20140922 DMD488 Cot1546.lif Field3 ch00.jpg
Field 3
- 20140922 DMD488 Cot1546.lif Zoom1 ch00.jpg
Zoom 1
These images don't tell me a great deal one way or another. There appears to be good dystrophin signal, so I think that the probes must be working, but it is difficult to check whether or not that signal is dystrophin itself. The Cot1 continues to perform admirably.
Chr21 Padlock Probes
- 20140922 PPRL488 Cot1546.lif Field1 NoThreshold ch00.jpg
No Threshold
- 20140922 PPRL488 Cot1546.lif Field1 NormalThreshold ch00.jpg
Normal Zero Threshold (20/255)
- 20140922 PPRL488 Cot1546.lif Field1 BestThreshold ch00.jpg
Best Threshold (100/255)
These images have much stronger green signal, corresponding to the Padlock Probes from Rui (PPRL). The comforting thing is that since there are thousands of these probes, I would expect a stronger signal. However, they are only supposed to paint chromosome 21, and even then not the whole thing. Therefore I should only see 2 or so concentrated dots, but I typically see much stronger signal than that. Tomorrow's microscopy may provide more insight.