Daniel:Notebook/HiResChrPaint/2014-10-2: Difference between revisions
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>Djacobse (Created page with "=Hybridization Buffer Test= Back to Calendar I'm going to run a FISH test on several different hybridization buffers, from several protocol...") |
>Djacobse No edit summary |
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I'm going to run a FISH test on several different hybridization buffers, from several protocols. | I'm going to run a FISH test on several different hybridization buffers, from several protocols. | ||
*Beliveau Buffer (Standard) | |||
**2X SSCT (0.1% Tween 20) | |||
**10% dextran sulfate | |||
**10 ug RNAse A | |||
**50% Formamide | |||
*Beliveau Buffer (No Tween) | |||
**2X SSC | **2X SSC | ||
**10% dextran sulfate | **10% dextran sulfate | ||
**10 ug RNAse A | **10 ug RNAse A | ||
**50% Formamide | **50% Formamide | ||
*Bienko Buffer | |||
** | **5X Denhardt's solution | ||
**1.7X SSC | |||
**50% Formamide | |||
**10% dextran sulfate | |||
**50 mM Na2HPO4/NaH2PO4 buffer | |||
*Raj Buffer | |||
**0.02% BSA | |||
**2X SSC | |||
**10% Formamide | |||
==Hybridization== | ==Hybridization== |
Revision as of 18:13, 2 October 2014
Hybridization Buffer Test
I'm going to run a FISH test on several different hybridization buffers, from several protocols.
- Beliveau Buffer (Standard)
- 2X SSCT (0.1% Tween 20)
- 10% dextran sulfate
- 10 ug RNAse A
- 50% Formamide
- Beliveau Buffer (No Tween)
- 2X SSC
- 10% dextran sulfate
- 10 ug RNAse A
- 50% Formamide
- Bienko Buffer
- 5X Denhardt's solution
- 1.7X SSC
- 50% Formamide
- 10% dextran sulfate
- 50 mM Na2HPO4/NaH2PO4 buffer
- Raj Buffer
- 0.02% BSA
- 2X SSC
- 10% Formamide