Daniel:Notebook/HiResChrPaint/2014-10-2: Difference between revisions

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(Created page with "=Hybridization Buffer Test= Back to Calendar I'm going to run a FISH test on several different hybridization buffers, from several protocol...")
 
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I'm going to run a FISH test on several different hybridization buffers, from several protocols.
I'm going to run a FISH test on several different hybridization buffers, from several protocols.


#Beliveau Buffer (Standard)
*Beliveau Buffer (Standard)
**2X SSCT (0.1% Tween 20)
**10% dextran sulfate
**10 ug RNAse A
**50% Formamide
*Beliveau Buffer (No Tween)
**2X SSC
**2X SSC
**10% dextran sulfate
**10% dextran sulfate
**10 ug RNAse A
**10 ug RNAse A
**50% Formamide
**50% Formamide
#Bienko Buffer
*Bienko Buffer
**
**5X Denhardt's solution
#Raj Buffer
**1.7X SSC
**50% Formamide
**10% dextran sulfate
**50 mM Na2HPO4/NaH2PO4 buffer
*Raj Buffer
**0.02% BSA
**2X SSC
**10% Formamide


==Hybridization==
==Hybridization==

Revision as of 18:13, 2 October 2014

Hybridization Buffer Test

Back to Calendar

I'm going to run a FISH test on several different hybridization buffers, from several protocols.

  • Beliveau Buffer (Standard)
    • 2X SSCT (0.1% Tween 20)
    • 10% dextran sulfate
    • 10 ug RNAse A
    • 50% Formamide
  • Beliveau Buffer (No Tween)
    • 2X SSC
    • 10% dextran sulfate
    • 10 ug RNAse A
    • 50% Formamide
  • Bienko Buffer
    • 5X Denhardt's solution
    • 1.7X SSC
    • 50% Formamide
    • 10% dextran sulfate
    • 50 mM Na2HPO4/NaH2PO4 buffer
  • Raj Buffer
    • 0.02% BSA
    • 2X SSC
    • 10% Formamide

Hybridization