Blue:RNA-Seq Experiments:10062014: Difference between revisions

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>B1lake
(Created page with "== Size Analysis of Mouse DRG Nuclei == *Recieved DRG nuclei (p2) from Lulu (Guoping Fan Lab, UCLA) on 10-03-2014 * Diluted nuclei to 600/ul, ran on biorad - one tight peak ...")
 
>B1lake
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* Checked under microscope - extensive clusters
* Checked under microscope - extensive clusters
* Filtered with 20um filter to clean up - removed many clusters - dropped [] to 250/ul
* Filtered with 20um filter to clean up - removed many clusters - dropped [] to 250/ul
* Measured nuclei width:
* Measured nuclei width using microscope (10x objective) and metamorph software:


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Revision as of 22:42, 6 October 2014

Size Analysis of Mouse DRG Nuclei

  • Recieved DRG nuclei (p2) from Lulu (Guoping Fan Lab, UCLA) on 10-03-2014
  • Diluted nuclei to 600/ul, ran on biorad - one tight peak at 5um
  • Checked under microscope - extensive clusters
  • Filtered with 20um filter to clean up - removed many clusters - dropped [] to 250/ul
  • Measured nuclei width using microscope (10x objective) and metamorph software:
Nuclei Width (um)
6.45
9.03
6.45
5.8
9.03
5.8
4.52
7.21
5.95
5.32
7.1
7.1
6.45
5.8
7.1
7.74
5.8
5.8
5.8
5.8
7.1
5.16
5.8
8.48
10.32
7.1
5.95
5.32
5.8
7.1
9.68
6.45
Mean = 6.6971875