Daniel:Notebook/HiResChrPaint/2014-10-4: Difference between revisions

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==Microscopy==
==Microscopy==


<gallery heights=600px widths=600px caption="DMD-488 (green) and Cot1-546 (red)" perrow=2>
===Beliveau Protocol, SSCT===
File:20141004_Believeau_SSCT.lif_Production1_ch00.jpg|Beliveau protocol, original hybridization buffer
<gallery heights=500px widths=500px caption="Original Protocol (Beliveau) DMD-488 (green) and Cot1-546 (red)" perrow=2>
File:20141004_Believeau_SSCT.lif_Production3_ch00.jpg|Beliveau protocol, original hybridization buffer
File:20141004_Believeau_SSCT.lif_Production1_ch00.jpg|Field 1
File:20141004_Beliveau.lif_Production1_ch00.jpg|Beliveau protocol, SSC buffer
File:20141004_Believeau_SSCT.lif_Production3_ch00.jpg|Field 2
File:20141004_Beliveau.lif_Production3_ch00.jpg|Beliveau protocol, SSC buffer
File:20141004_Bienko.lif_Production1_ch00.jpg|Bienko protocol
File:20141004_Bienko.lif_Production3_ch00.jpg|Bienko protocol
File:20141004_Raj.lif_Production1_ch00.jpg|Raj protocol
File:20141004_Raj.lif_Production2_ch00.jpg|Raj protocol
</gallery>
</gallery>
===Beliveau Protocol, SSC===
<gallery heights=500px widths=500px caption="Beliveau Protocol with SSC DMD-488 (green) and Cot1-546 (red)" perrow=2>
File:20141004_Beliveau.lif_Production1_ch00.jpg|Field 1
File:20141004_Beliveau.lif_Production3_ch00.jpg|Field 2
</gallery>
===Bienko Protocol===
<gallery heights=500px widths=500px caption="Bienko protcol DMD-488 (green) and Cot1-546 (red)" perrow=2>
File:20141004_Bienko.lif_Production1_ch00.jpg|Field 1
File:20141004_Bienko.lif_Production3_ch00.jpg|Field 2
</gallery>
===Raj Protocol===
<gallery heights=500px widths=500px caption="Raj Protocol DMD-488 (green) and Cot1-546 (red)" perrow=2>
File:20141004_Raj.lif_Production1_ch00.jpg|Field 1
File:20141004_Raj.lif_Production2_ch00.jpg|Field 2
</gallery>
==Analysis==
The Cot1 seems to be the same for all 4 samples.  As for the DMD signal, it appears to be the best with the Beliveau SSC and the Bienko protocols, although it is difficult to say since all 4 protocols have some nuclear signal.  All 4 have some nucleii with 2 signals, some with greater than 2, some with less than 2.  But the Beliveau SSC and Bienko have the most consistent nucleii.  Still, without more rigorous analysis (MATLAB), it is difficult to tell ultimately which method worked the best.
[[Category:HRCP]] [[Category:Microscopy]] [[Category:20141002]]

Latest revision as of 21:49, 4 November 2014

Hybridization Buffer Test (Started 10/2/2014)[edit]

Back to Calendar

Microscopy[edit]

Beliveau Protocol, SSCT[edit]

Beliveau Protocol, SSC[edit]

Bienko Protocol[edit]

Raj Protocol[edit]

Analysis[edit]

The Cot1 seems to be the same for all 4 samples. As for the DMD signal, it appears to be the best with the Beliveau SSC and the Bienko protocols, although it is difficult to say since all 4 protocols have some nuclear signal. All 4 have some nucleii with 2 signals, some with greater than 2, some with less than 2. But the Beliveau SSC and Bienko have the most consistent nucleii. Still, without more rigorous analysis (MATLAB), it is difficult to tell ultimately which method worked the best.