Hosuk:LabNotes/2014-10-20: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Hosuki78
No edit summary
>Hosuki78
No edit summary
 
(5 intermediate revisions by the same user not shown)
Line 6: Line 6:
*From [[Hosuk:LabNotes/2014-10-15|2014-10-15]]
*From [[Hosuk:LabNotes/2014-10-15|2014-10-15]]


*1st Rolonies
 
*[[File:DecodingPGP1F_Agi0gap-0gapSupp-20gap_1stRolonies.png|600px]]
====Result====
*Suppressor worked well
*Decoded genes were not many
*20gap didn’t make 2nd Rolonies well
 
 
*For the next plan
**Decode again with PGP1F-0gap, and 0gap with suppressor, I need to get more number of detected features
**So, I'll re-generate 2nd Rolonies and decode again
 
 
 
====Result - Image and Data====
 
*1st Rolonies - good in each dish
*[[File:DecodingPGP1F_Agi0gap-0gapSupp-20gap_1stRolonies.png|800px]]
 
 
 
*2nd Rolony images by  maximum projected with all 7 cycle images
**Not as many as I expected...
** Too few 2nd Rolony detected from 20gap
 
 
*[[File:DecodingPGP1F_Agi0gap-0gapSupp-20gap_2ndRolonies.png|600px]]
 
 
 
*Intensity Distribution
*[[File:DecodingPGP1F_Agi0gap-0gapSupp-20gap_ColorDistribution.png|600px]]
 
 
*Gene Mapping
*[[File:DecodingPGP1F_Agi0gap-0gapSupp-20gap_GeneMapping.png|600px]]
 
 
*Comparison between data - previous decoding data(decoded at 09/05), current, FISSEQ from Jay, and Gene expression from Illumina
*[[File:DecodingPGP1F_Agi0gap-0gapSupp-20gap_CompareGeneData.png|800px]]

Latest revision as of 19:17, 20 October 2014


Decoding PGP1F - Agi26k 20gap, 0gap, 0gap with Suppressor[edit]

  • Decoding PGP1F with different Padlock Probes
  • From 2014-10-15


Result[edit]

  • Suppressor worked well
  • Decoded genes were not many
  • 20gap didn’t make 2nd Rolonies well


  • For the next plan
    • Decode again with PGP1F-0gap, and 0gap with suppressor, I need to get more number of detected features
    • So, I'll re-generate 2nd Rolonies and decode again


Result - Image and Data[edit]


  • 2nd Rolony images by maximum projected with all 7 cycle images
    • Not as many as I expected...
    • Too few 2nd Rolony detected from 20gap