Hosuk:LabNotes/2014-10-20: Difference between revisions
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*Decoding PGP1F with different Padlock Probes | *Decoding PGP1F with different Padlock Probes | ||
*From [[Hosuk:LabNotes/2014-10-15|2014-10-15]] | *From [[Hosuk:LabNotes/2014-10-15|2014-10-15]] | ||
====Result==== | |||
*Suppressor worked well | |||
*Decoded genes were not many | |||
*20gap didn’t make 2nd Rolonies well | |||
*For the next plan | |||
**Decode again with PGP1F-0gap, and 0gap with suppressor, I need to get more number of detected features | |||
**So, I'll re-generate 2nd Rolonies and decode again | |||
====Result - Image and Data==== | |||
*1st Rolonies - good in each dish | *1st Rolonies - good in each dish | ||
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*[[File:DecodingPGP1F_Agi0gap-0gapSupp-20gap_2ndRolonies.png| | *[[File:DecodingPGP1F_Agi0gap-0gapSupp-20gap_2ndRolonies.png|600px]] | ||
*Intensity Distribution | |||
*[[File:DecodingPGP1F_Agi0gap-0gapSupp-20gap_ColorDistribution.png|600px]] | |||
*Gene Mapping | |||
*[[File:DecodingPGP1F_Agi0gap-0gapSupp-20gap_GeneMapping.png|600px]] | |||
*Comparison between data - previous decoding data(decoded at 09/05), current, FISSEQ from Jay, and Gene expression from Illumina | |||
*[[File:DecodingPGP1F_Agi0gap-0gapSupp-20gap_CompareGeneData.png|800px]] |
Latest revision as of 19:17, 20 October 2014
Decoding PGP1F - Agi26k 20gap, 0gap, 0gap with Suppressor[edit]
- Decoding PGP1F with different Padlock Probes
- From 2014-10-15
Result[edit]
- Suppressor worked well
- Decoded genes were not many
- 20gap didn’t make 2nd Rolonies well
- For the next plan
- Decode again with PGP1F-0gap, and 0gap with suppressor, I need to get more number of detected features
- So, I'll re-generate 2nd Rolonies and decode again
Result - Image and Data[edit]
- 1st Rolonies - good in each dish
- File:DecodingPGP1F Agi0gap-0gapSupp-20gap 1stRolonies.png
- 2nd Rolony images by maximum projected with all 7 cycle images
- Not as many as I expected...
- Too few 2nd Rolony detected from 20gap
- Intensity Distribution
- File:DecodingPGP1F Agi0gap-0gapSupp-20gap ColorDistribution.png
- Comparison between data - previous decoding data(decoded at 09/05), current, FISSEQ from Jay, and Gene expression from Illumina
- File:DecodingPGP1F Agi0gap-0gapSupp-20gap CompareGeneData.png