Daniel:CellLines/U87MG: Difference between revisions
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[[Daniel Jacobsen|Back to Main]] | [[Daniel Jacobsen|Back to Main]] | ||
[http://www. | [http://www.atcc.org/products/all/HTB-14.aspx ATCC Website] | ||
''' | '''Morphology''': Epithelial Cell | ||
'''Cell Line Description''': | '''Cell Line Description''': This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found. | ||
'''Media''':EMEM (EBSS | '''Media''':EMEM (EBSS) | ||
*10% Fetal Bovine Serum (FBS) | *10% Fetal Bovine Serum (FBS) | ||
*1% non-essential amino acids | |||
*2 mM L-glutamine | |||
*1 mM sodium pyruvate | |||
*1500 mg/L sodium bicarbonate |
Revision as of 00:30, 8 November 2014
U-87MG Cell Line
Morphology: Epithelial Cell
Cell Line Description: This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found.
Media:EMEM (EBSS)
- 10% Fetal Bovine Serum (FBS)
- 1% non-essential amino acids
- 2 mM L-glutamine
- 1 mM sodium pyruvate
- 1500 mg/L sodium bicarbonate