Daniel:CellLines/U87MG: Difference between revisions
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''' | [http://www.atcc.org/products/all/HTB-14.aspx ATCC Website] | ||
[http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM587180 Gene Expression (NCBI)] | |||
'''Morphology''': Epithelial Cell | |||
'''Cell Line Description''': This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found. | |||
'''Media''':EMEM (EBSS) | |||
*10% Fetal Bovine Serum (FBS) | *10% Fetal Bovine Serum (FBS) | ||
*1% non-essential amino acids | |||
*2 mM L-glutamine | |||
*1 mM sodium pyruvate | |||
*1500 mg/L sodium bicarbonate |
Latest revision as of 23:18, 8 December 2014
U-87MG Cell Line[edit]
Morphology: Epithelial Cell
Cell Line Description: This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found.
Media:EMEM (EBSS)
- 10% Fetal Bovine Serum (FBS)
- 1% non-essential amino acids
- 2 mM L-glutamine
- 1 mM sodium pyruvate
- 1500 mg/L sodium bicarbonate