Blue:RNA-Seq Experiments:12012014: Difference between revisions
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==LiuKai1-4 Plates: NexteraXT Library Preparation== | |||
*Repeated Nextera on Normalized Samples (note: First attempt showed smears above 800bp, indicating bad stock of transposase - repeated with fresh stock) | |||
*Gel purified PCR products twice with 0.8x beads and resuspended in 10ul for each library: | |||
**LiuKai_1: Nu-iPSC early stage cells | |||
**LiuKai_2: Nu-iPSC late stage cellss | |||
**LiuKai_3: Normal iPSC early stage cells | |||
**LiuKai_4: Normal iPSC late stage cells | |||
Note: cDNA samples chosen for further processing decided by Liu Kai | |||
Ran 1ul of each library on gel: | |||
[[File:12012014_LiuKai_Miseq.jpg]] | [[File:12012014_LiuKai_Miseq.jpg]] |
Revision as of 02:09, 2 December 2014
LiuKai1-4 Plates: NexteraXT Library Preparation
- Repeated Nextera on Normalized Samples (note: First attempt showed smears above 800bp, indicating bad stock of transposase - repeated with fresh stock)
- Gel purified PCR products twice with 0.8x beads and resuspended in 10ul for each library:
- LiuKai_1: Nu-iPSC early stage cells
- LiuKai_2: Nu-iPSC late stage cellss
- LiuKai_3: Normal iPSC early stage cells
- LiuKai_4: Normal iPSC late stage cells
Note: cDNA samples chosen for further processing decided by Liu Kai
Ran 1ul of each library on gel: