Blue:RNA-Seq Experiments:12012014: Difference between revisions
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==LiuKai1-4 Plates: NexteraXT Library Preparation== | ==LiuKai1-4 Plates: NexteraXT Library Preparation== | ||
*Repeated Nextera on Normalized Samples (note: First attempt showed smears above 800bp, indicating bad stock of transposase - repeated with fresh stock) | *Repeated Nextera on Normalized Samples prepared by Derek (note: First attempt at Nextera showed smears above 800bp, indicating bad stock of transposase - repeated with fresh stock) | ||
*Gel purified PCR products twice with 0.8x beads and resuspended in 10ul for each library: | *Gel purified PCR products twice with 0.8x beads and resuspended in 10ul for each library: | ||
**LiuKai_1: Nu-iPSC early stage cells | **LiuKai_1: Nu-iPSC early stage cells | ||
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**LiuKai_4: Normal iPSC late stage cells | **LiuKai_4: Normal iPSC late stage cells | ||
Note: cDNA samples chosen for further processing decided by Liu Kai | Note: The subset of cDNA samples chosen for further processing was decided by Liu Kai | ||
Ran 1ul of each library on gel: | Ran 1ul of each library on gel: | ||
[[File:12012014_LiuKai_Miseq.jpg]] | [[File:12012014_LiuKai_Miseq.jpg]] |
Latest revision as of 02:10, 2 December 2014
LiuKai1-4 Plates: NexteraXT Library Preparation[edit]
- Repeated Nextera on Normalized Samples prepared by Derek (note: First attempt at Nextera showed smears above 800bp, indicating bad stock of transposase - repeated with fresh stock)
- Gel purified PCR products twice with 0.8x beads and resuspended in 10ul for each library:
- LiuKai_1: Nu-iPSC early stage cells
- LiuKai_2: Nu-iPSC late stage cellss
- LiuKai_3: Normal iPSC early stage cells
- LiuKai_4: Normal iPSC late stage cells
Note: The subset of cDNA samples chosen for further processing was decided by Liu Kai
Ran 1ul of each library on gel: