Noi:Labnote tracking: Difference between revisions

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  '''Date                        Description'''
  '''Date                        Description'''
  '''December 2014'''
  '''December 2014'''
  14-12-11                     RRBS library prep, mouse hippocampus gDNA (Zhou's lab)
  2014-12-11                   RRBS library prep, mouse hippocampus gDNA (Zhou's lab)
  14-12-08                     LMS + CRC BSPP capture on WGBS libraries prepared from ctDNA
  2014-12-08                   LMS + CRC BSPP capture on WGBS libraries prepared from ctDNA
                               of CRC patient (Illumina)
                               of CRC patient (Illumina)
  14-12-05                     WGBS library prep and BSPP capture of cfDNA from Illumina (with Li Liu)
  2014-12-05                   WGBS library prep and BSPP capture of cfDNA from Illumina (with Li Liu)
                               ctDNA of colorectal cancer (CRC) patients from Illumina  
                               ctDNA of colorectal cancer (CRC) patients from Illumina  
                               (samples from Kansas University)
                               (samples from Kansas University)
  14-12-04                     SeqCap Epi probe pool (UMR v1) hybridization capture on cancer patient
  2014-12-04                   SeqCap Epi probe pool (UMR v1) hybridization capture on cancer patient
                               WGBS libraries (plasma/tumor tissue DNA, )
                               WGBS libraries (plasma/tumor tissue DNA, )
                               2nd experiment (used 3 tubes of LMS SeqCap probe pool)
                               2nd experiment (used 3 tubes of LMS SeqCap probe pool)


  '''November 2014'''
  '''November 2014'''
  14-11-25                     Comparison of the efficiency between TruSeq adaptor and a customized  
  2014-11-25                   Comparison of the efficiency between TruSeq adaptor and a customized  
                               unmethylated Y-adaptor with UMI
                               unmethylated Y-adaptor with UMI
  [[Noi/NOTES/2014-11-4|14-12-04]]                    SeqCap Epi probe pool (UMR v1) hybridization capture on normal and cancer  
  [[Noi/NOTES/2014-11-4|14-12-04]]                    SeqCap Epi probe pool (UMR v1) hybridization capture  
                              patient WGBS libraries  
                              on normal and cancer patient WGBS libraries  
                               1st Experiment (used 2 tubes of LMS SeqCap probe pool)
                               1st Experiment (used 2 tubes of LMS SeqCap probe pool)
                               <u>Also included</u>  
                               <u>Also included</u>  
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  '''October 2014'''
  '''October 2014'''
  14-10-31                     Repeating of BIS/DNA SMART ChIP-Seq protocol
  2014-10-31                   Repeating of BIS/DNA SMART ChIP-Seq protocol
  14-10-29                     Generation of sequencing library of a customized SeqCap EZ Epi Probe Pool to decode the sequences
  2014-10-29                   Generation of sequencing library of a customized SeqCap EZ Epi Probe Pool  
                              of LMS SeqCap probe pool
                              to decode the sequences of LMS SeqCap probe pool
                               Library generated by DNA SMART ChIP Seq Kit
                               Library generated by DNA SMART ChIP Seq Kit
2014-10-24                  Preparation of WGBS of cfDNA from plasma using KAPA Hyper Prep kit &
                              NEXTflex Cell Free DNA-Seq kit
                              To compare the efficiency between the two commercial kits
                              Repeated experiment of 14-1022
2014-10-22                  Preparation of WGBS of cfDNA from plasma using KAPA Hyper Prep kit
2014-10-15                  Expansion PCR of new probe set from CustomArrays (90k_oligos_30Sept2014)
                              Bad oligos. Got re-synthesized of the new oligos by CustomArrays
                              The new tube was amplified by [[Rui:LabNotes/Haplotyping/2014-11-12| Rui on 14-11-12]]
2014-10-08                  Integration of DNA SMART ChIP-Seq protocol with RRBS for low input DNA methylation assay

Revision as of 12:41, 24 December 2014

Labnote tracking

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Date                         Description
December 2014
2014-12-11                   RRBS library prep, mouse hippocampus gDNA (Zhou's lab)
2014-12-08                   LMS + CRC BSPP capture on WGBS libraries prepared from ctDNA
                             of CRC patient (Illumina)
2014-12-05                   WGBS library prep and BSPP capture of cfDNA from Illumina (with Li Liu)
                             ctDNA of colorectal cancer (CRC) patients from Illumina 
                             (samples from Kansas University)
2014-12-04                   SeqCap Epi probe pool (UMR v1) hybridization capture on cancer patient
                             WGBS libraries (plasma/tumor tissue DNA, )
                             2nd experiment (used 3 tubes of LMS SeqCap probe pool)
November 2014
2014-11-25                   Comparison of the efficiency between TruSeq adaptor and a customized 
                             unmethylated Y-adaptor with UMI
14-12-04                     SeqCap Epi probe pool (UMR v1) hybridization capture 
                             on normal and cancer patient WGBS libraries 
                             1st Experiment (used 2 tubes of LMS SeqCap probe pool)
                             Also included 
                             Part I: Re-amplification of WGBS libraries of normal plasma samples
October 2014
2014-10-31                   Repeating of BIS/DNA SMART ChIP-Seq protocol
2014-10-29                   Generation of sequencing library of a customized SeqCap EZ Epi Probe Pool 
                             to decode the sequences of LMS SeqCap probe pool
                             Library generated by DNA SMART ChIP Seq Kit
2014-10-24                   Preparation of WGBS of cfDNA from plasma using KAPA Hyper Prep kit & 
                             NEXTflex Cell Free DNA-Seq kit
                             To compare the efficiency between the two commercial kits
                             Repeated experiment of 14-1022
2014-10-22                   Preparation of WGBS of cfDNA from plasma using KAPA Hyper Prep kit
2014-10-15                   Expansion PCR of new probe set from CustomArrays (90k_oligos_30Sept2014)
                             Bad oligos. Got re-synthesized of the new oligos by CustomArrays 
                             The new tube was amplified by  Rui on 14-11-12
2014-10-08                   Integration of DNA SMART ChIP-Seq protocol with RRBS for low input DNA methylation assay