Arichard:Notebook/bulk150210: Difference between revisions

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>Andrew
>Andrew
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Following protocol from [http://genome-tech.ucsd.edu/LabNotes/index.php/Arichard:Notebook/bulk150122#Bulk_libraries_January_22.2C_2015a January 22]
Following protocol from [http://genome-tech.ucsd.edu/LabNotes/index.php/Arichard:Notebook/bulk150122#Bulk_libraries_January_22.2C_2015a January 22]
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''sort'''
| align="center" style="background:#f0f0f0;"|'''# on tube'''
| align="center" style="background:#f0f0f0;"|'''nuclei/ul'''
| align="center" style="background:#f0f0f0;"|'''ul/tube'''
| align="center" style="background:#f0f0f0;"|'''nuclei/sample'''
| align="center" style="background:#f0f0f0;"|'''# samples'''
| align="center" style="background:#f0f0f0;"|'''# tubes to pool'''
| align="center" style="background:#f0f0f0;"|'''ul/sample'''
| align="center" style="background:#f0f0f0;"|'''ul HLS'''
|-
| 1-02 ctx full||1||120||3||1000||1||3||8.33||26.67
|-
| 1-02 ctx hi||2||120||3||1000||1||3||8.33||26.67
|-
| 1-02 ctx lo||3||78||10||1000||1||2||12.82||22.18
|-
| 1-02 cbl full||4||274||10||1000||3||2||3.65||31.35
|-
| 25-01 ctx full||5||283||20||1000||3||1||3.53||31.47
|-
| 25-01 cbl full||6||232||10||1000||3||2||4.31||30.69
|-
|
|}

Revision as of 18:48, 10 February 2015

February 10, 2015 bulk Nextera on 1-02 and 25-01

Samples

from October 09, 2014

Following protocol from January 22

sort # on tube nuclei/ul ul/tube nuclei/sample # samples # tubes to pool ul/sample ul HLS
1-02 ctx full 1 120 3 1000 1 3 8.33 26.67
1-02 ctx hi 2 120 3 1000 1 3 8.33 26.67
1-02 ctx lo 3 78 10 1000 1 2 12.82 22.18
1-02 cbl full 4 274 10 1000 3 2 3.65 31.35
25-01 ctx full 5 283 20 1000 3 1 3.53 31.47
25-01 cbl full 6 232 10 1000 3 2 4.31 30.69