Arichard:Notebook/bulk150210: Difference between revisions
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Following protocol from [http://genome-tech.ucsd.edu/LabNotes/index.php/Arichard:Notebook/bulk150122#Bulk_libraries_January_22.2C_2015a January 22] | Following protocol from [http://genome-tech.ucsd.edu/LabNotes/index.php/Arichard:Notebook/bulk150122#Bulk_libraries_January_22.2C_2015a January 22] | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''sort''' | |||
| align="center" style="background:#f0f0f0;"|'''# on tube''' | |||
| align="center" style="background:#f0f0f0;"|'''nuclei/ul''' | |||
| align="center" style="background:#f0f0f0;"|'''ul/tube''' | |||
| align="center" style="background:#f0f0f0;"|'''nuclei/sample''' | |||
| align="center" style="background:#f0f0f0;"|'''# samples''' | |||
| align="center" style="background:#f0f0f0;"|'''# tubes to pool''' | |||
| align="center" style="background:#f0f0f0;"|'''ul/sample''' | |||
| align="center" style="background:#f0f0f0;"|'''ul HLS''' | |||
|- | |||
| 1-02 ctx full||1||120||3||1000||1||3||8.33||26.67 | |||
|- | |||
| 1-02 ctx hi||2||120||3||1000||1||3||8.33||26.67 | |||
|- | |||
| 1-02 ctx lo||3||78||10||1000||1||2||12.82||22.18 | |||
|- | |||
| 1-02 cbl full||4||274||10||1000||3||2||3.65||31.35 | |||
|- | |||
| 25-01 ctx full||5||283||20||1000||3||1||3.53||31.47 | |||
|- | |||
| 25-01 cbl full||6||232||10||1000||3||2||4.31||30.69 | |||
|- | |||
| | |||
|} |
Revision as of 18:48, 10 February 2015
February 10, 2015 bulk Nextera on 1-02 and 25-01
Samples
Following protocol from January 22
sort | # on tube | nuclei/ul | ul/tube | nuclei/sample | # samples | # tubes to pool | ul/sample | ul HLS |
1-02 ctx full | 1 | 120 | 3 | 1000 | 1 | 3 | 8.33 | 26.67 |
1-02 ctx hi | 2 | 120 | 3 | 1000 | 1 | 3 | 8.33 | 26.67 |
1-02 ctx lo | 3 | 78 | 10 | 1000 | 1 | 2 | 12.82 | 22.18 |
1-02 cbl full | 4 | 274 | 10 | 1000 | 3 | 2 | 3.65 | 31.35 |
25-01 ctx full | 5 | 283 | 20 | 1000 | 3 | 1 | 3.53 | 31.47 |
25-01 cbl full | 6 | 232 | 10 | 1000 | 3 | 2 | 4.31 | 30.69 |