Arichard:Notebook/midas150130: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Andrew
No edit summary
>Andrew
No edit summary
Line 3: Line 3:
[[File:midas150130Slide2.jpg|400px]]
[[File:midas150130Slide2.jpg|400px]]


I then tried 1X Tn5059 on strip 2
[[File:midas150130Slide3.jpg|400px]]
[[File:midas150130Slide3.jpg|400px]]
[[File:midas150130Slide4.jpg|400px]]
[[File:midas150130Slide4.jpg|400px]]

Revision as of 19:36, 2 March 2015

Used old 1:50 Nextera on first strip File:Midas150130Slide1.jpg File:Midas150130Slide2.jpg

I then tried 1X Tn5059 on strip 2 File:Midas150130Slide3.jpg File:Midas150130Slide4.jpg

Not sure why Tn5059 is not working on these MIDAS amplicons. It may have just been an unlucky batch. I repeated with the remaining samples.

File:Midas150130Slide5.jpg File:Midas150130Slide6.jpg File:Midas150130Slide7.jpg File:Midas150130Slide8.jpg

It looked like these ones worked, so I had Qiming go ahead with bead purification.

File:Midas150130Slide9.jpg File:Midas150130Slide10.jpg File:Midas150130Slide11.jpg File:Midas150130Slide12.jpg