Arichard:Notebook/midas150130: Difference between revisions

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==January 1, 2015==
MIDAS on 1-02 (AD) and 25-01 (CTL)
===Gels===
Used old 1:50 Nextera on first strip
Used old 1:50 Nextera on first strip
[[File:midas150130Slide1.jpg|400px]]
[[File:midas150130Slide1.jpg|400px]]
[[File:midas150130Slide2.jpg|400px]]
[[File:midas150130Slide2.jpg|400px]]


I then tried 1X Tn5059 on strip 2
[[File:midas150130Slide3.jpg|400px]]
[[File:midas150130Slide3.jpg|400px]]
[[File:midas150130Slide4.jpg|400px]]
[[File:midas150130Slide4.jpg|400px]]
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[[File:midas150130Slide11.jpg|400px]]
[[File:midas150130Slide11.jpg|400px]]
[[File:midas150130Slide12.jpg|400px]]
[[File:midas150130Slide12.jpg|400px]]
===Sequencing===
Submitted:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Array/Sample #'''
| align="center" style="background:#f0f0f0;"|'''Patient'''
| align="center" style="background:#f0f0f0;"|'''NeuN'''
| align="center" style="background:#f0f0f0;"|'''Tissue'''
| align="center" style="background:#f0f0f0;"|'''PI peak'''
| align="center" style="background:#f0f0f0;"|'''Sort date'''
|-
| A2||1-02||+||Cortex||Full 2N||9-Oct-14
|-
| A3||1-02||+||Cortex||Full 2N||9-Oct-14
|-
| A4||1-02||+||Cortex||Full 2N||9-Oct-14
|-
| C19||1-02||+||Cortex||Full 2N||9-Oct-14
|-
| C20||1-02||+||Cortex||Full 2N||9-Oct-14
|-
| C21||1-02||+||Cortex||Full 2N||9-Oct-14
|-
| J33||25-01||+||Cortex||Full 2N||9-Oct-14
|-
| J35||25-01||+||Cortex||Full 2N||9-Oct-14
|-
| K41||25-01||+||Cortex||Full 2N||9-Oct-14
|-
| K44||25-01||+||Cortex||Full 2N||9-Oct-14
|-
|
|}

Latest revision as of 19:43, 2 March 2015

January 1, 2015[edit]

MIDAS on 1-02 (AD) and 25-01 (CTL)

Gels[edit]

Used old 1:50 Nextera on first strip File:Midas150130Slide1.jpg File:Midas150130Slide2.jpg

I then tried 1X Tn5059 on strip 2 File:Midas150130Slide3.jpg File:Midas150130Slide4.jpg

Not sure why Tn5059 is not working on these MIDAS amplicons. It may have just been an unlucky batch. I repeated with the remaining samples.

File:Midas150130Slide5.jpg File:Midas150130Slide6.jpg File:Midas150130Slide7.jpg File:Midas150130Slide8.jpg

It looked like these ones worked, so I had Qiming go ahead with bead purification.

File:Midas150130Slide9.jpg File:Midas150130Slide10.jpg File:Midas150130Slide11.jpg File:Midas150130Slide12.jpg

Sequencing[edit]

Submitted:

Array/Sample # Patient NeuN Tissue PI peak Sort date
A2 1-02 + Cortex Full 2N 9-Oct-14
A3 1-02 + Cortex Full 2N 9-Oct-14
A4 1-02 + Cortex Full 2N 9-Oct-14
C19 1-02 + Cortex Full 2N 9-Oct-14
C20 1-02 + Cortex Full 2N 9-Oct-14
C21 1-02 + Cortex Full 2N 9-Oct-14
J33 25-01 + Cortex Full 2N 9-Oct-14
J35 25-01 + Cortex Full 2N 9-Oct-14
K41 25-01 + Cortex Full 2N 9-Oct-14
K44 25-01 + Cortex Full 2N 9-Oct-14