Daniel:Notebook/RNAFACS/2015-4-11: Difference between revisions

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>Djacobse
(Created page with "=In Tube FISH Test (Started 4/10/2015) = Back to Calendar ==Dye Labeling== #Centrifuge at 4 C for 30 minut...")
 
>Djacobse
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#Dry the pellet in the hood  
#Dry the pellet in the hood  
#Resuspend pellet in 12 uL TE
#Resuspend pellet in 12 uL TE
==Hybridization==
#Resuspend cells in RPHB with labeled probes (0.5-1ng/uL)
#Incubate overnight at 30C

Revision as of 20:26, 11 April 2015

In Tube FISH Test (Started 4/10/2015)

Back to Calendar

Dye Labeling

  1. Centrifuge at 4 C for 30 minutes (14000 rpm)
  2. Remove supernatant and add 750 uL chilled 75% EtOH
  3. Centrifuge at minutes at 4 C
  4. Dry the pellet in the hood
  5. Resuspend pellet in 12 uL TE

Hybridization

  1. Resuspend cells in RPHB with labeled probes (0.5-1ng/uL)
  2. Incubate overnight at 30C