Daniel:Notebook/RNAFACS/2015-4-11: Difference between revisions
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>Djacobse (Created page with "=In Tube FISH Test (Started 4/10/2015) = Back to Calendar ==Dye Labeling== #Centrifuge at 4 C for 30 minut...") |
>Djacobse |
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#Dry the pellet in the hood | #Dry the pellet in the hood | ||
#Resuspend pellet in 12 uL TE | #Resuspend pellet in 12 uL TE | ||
==Hybridization== | |||
#Resuspend cells in RPHB with labeled probes (0.5-1ng/uL) | |||
#Incubate overnight at 30C |
Revision as of 20:26, 11 April 2015
In Tube FISH Test (Started 4/10/2015)
Dye Labeling
- Centrifuge at 4 C for 30 minutes (14000 rpm)
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge at minutes at 4 C
- Dry the pellet in the hood
- Resuspend pellet in 12 uL TE
Hybridization
- Resuspend cells in RPHB with labeled probes (0.5-1ng/uL)
- Incubate overnight at 30C