CRISPR screen and single-cell sequencing project: Difference between revisions
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>YanWu (Created page with "The goal of this project is to screen H9-GFP tagged hESCs with 4 curated libraries and then use single-cell sequencing to determine which gRNA was incorporated into which cell...") |
>YanWu No edit summary |
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The goal of this project is to screen H9-GFP tagged hESCs with 4 curated libraries and then use single-cell sequencing to determine which gRNA was incorporated into which cell and gene expression in each cell. The four libraries and some annotation info is listed [[File:Crispr_screen_single_cell_sequencing_library.xlsx|here]]. | The goal of this project is to screen H9-GFP tagged hESCs with 4 curated libraries and then use single-cell sequencing to determine which gRNA was incorporated into which cell and gene expression in each cell. The four libraries and some annotation info is listed [[File:Crispr_screen_single_cell_sequencing_library.xlsx | here]]. | ||
Library Design: | Library Design: | ||
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Revision as of 00:18, 6 May 2015
The goal of this project is to screen H9-GFP tagged hESCs with 4 curated libraries and then use single-cell sequencing to determine which gRNA was incorporated into which cell and gene expression in each cell. The four libraries and some annotation info is listed here.
Library Design: