CRISPR screen and single-cell sequencing project: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>YanWu
No edit summary
>YanWu
No edit summary
Line 1: Line 1:
The goal of this project is to screen H9-GFP tagged hESCs with 4 curated libraries and then use single-cell sequencing to determine which gRNA was incorporated into which cell and gene expression in each cell. The four libraries and some annotation info is listed [[File:Crispr_screen_single_cell_sequencing_library.xlsx | here]].
The goal of this project is to screen H9-GFP tagged hESCs with 4 curated libraries and then use single-cell sequencing to determine which gRNA was incorporated into which cell and gene expression in each cell. The four libraries and some annotation info is listed [[File:Crispr_screen_single_cell_sequencing_library.xlsx]|here].


Library Design:
Library Design:
#
#

Revision as of 00:18, 6 May 2015

The goal of this project is to screen H9-GFP tagged hESCs with 4 curated libraries and then use single-cell sequencing to determine which gRNA was incorporated into which cell and gene expression in each cell. The four libraries and some annotation info is listed [[File:Crispr_screen_single_cell_sequencing_library.xlsx]|here].

Library Design: