Sam:LabNotes/Microbione/2009-3-3/exp1: Difference between revisions
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>Sam Chiang |
>Sam Chiang |
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===Slide washing=== | ===Slide washing (Tween 20/PBS washing) === | ||
#Add 20 uL of Tween 20 to 180 uL of H20 (10% dilution). | |||
#Add 19.2 mL of PBS to the solution for a final dilution of .1% Tween 20. | |||
#Shake until a soapy consistency is observed | |||
#Gently spray 5 ml of the solution over the microarrays | |||
#Wipe carefully with a kimwipe and allow to dry | |||
===Beads loading=== | ===Beads loading=== |
Revision as of 17:45, 5 March 2009
Slide washing (Tween 20/PBS washing)
- Add 20 uL of Tween 20 to 180 uL of H20 (10% dilution).
- Add 19.2 mL of PBS to the solution for a final dilution of .1% Tween 20.
- Shake until a soapy consistency is observed
- Gently spray 5 ml of the solution over the microarrays
- Wipe carefully with a kimwipe and allow to dry
Beads loading
Microscope image
- Beads in 1/100 dilution, objective 20X (left); Beads in 1/100 dilution, objective 10X (right)
File:Bead-3-4-09 1-100 2.bmp File:Bead-3-4-09 1-100.bmp
- Beads in 1/200 dilution, objective 20X (left); Beads in 1/200 dilution, objective 20X (right)
File:Bead-3-4-09 1-200 2.bmp File:Bead-3-4-09 1-200.bmp