CRISPR screen and single-cell sequencing project: Difference between revisions

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The goal of this project is to screen H9-GFP tagged hESCs with 4 curated libraries and then use single-cell sequencing to determine which gRNA was incorporated into which cell and gene expression in each cell. The four libraries and some annotation info is listed [[here|File:Crispr_screen_single_cell_sequencing_library.xlsx]].
The goal of this project is to screen H9-GFP tagged hESCs with 4 curated libraries and then use single-cell sequencing to determine which gRNA was incorporated into which cell and gene expression in each cell. The four libraries and some annotation info is listed [[File:Crispr_screen_single_cell_sequencing_library.xlsx]].


Library Design:
Library Design:
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Revision as of 00:19, 6 May 2015

The goal of this project is to screen H9-GFP tagged hESCs with 4 curated libraries and then use single-cell sequencing to determine which gRNA was incorporated into which cell and gene expression in each cell. The four libraries and some annotation info is listed File:Crispr screen single cell sequencing library.xlsx.

Library Design: