Sam:LabNotes/Microbione/2009-3-3/exp1: Difference between revisions

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>Sam Chiang
>Sam Chiang
Line 13: Line 13:
#Firmly press down until the squares are sealed
#Firmly press down until the squares are sealed
#Remove the protective covering from the other side and press down, ensuring a tight seal
#Remove the protective covering from the other side and press down, ensuring a tight seal


===Microscope image===
===Microscope image===

Revision as of 17:45, 5 March 2009

Slide washing (Tween 20/PBS washing)

  1. Add 20 uL of Tween 20 to 180 uL of H20 (10% dilution).
  2. Add 19.2 mL of PBS to the solution for a final dilution of .1% Tween 20.
  3. Shake until a soapy consistency is observed
  4. Gently spray 5 ml of the solution over the microarrays
  5. Wipe carefully with a kimwipe and allow to dry


Beads loading

  1. Remove the protective covering from one side of the gasket and place in the desired position on the microarray
  2. Firmly press down until the squares are sealed
  3. Remove the protective covering from the other side and press down, ensuring a tight seal


Microscope image

  • Beads in 1/100 dilution, objective 20X (left); Beads in 1/100 dilution, objective 10X (right)
  File:Bead-3-4-09 1-100 2.bmp    File:Bead-3-4-09 1-100.bmp 


  • Beads in 1/200 dilution, objective 20X (left); Beads in 1/200 dilution, objective 20X (right)
  File:Bead-3-4-09 1-200 2.bmp    File:Bead-3-4-09 1-200.bmp