Matt:LabNotes/2015-6-5: Difference between revisions

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>Mzcai
(Created page with "==RNA FISH: Probe Resuspension and Dye Coupling== ===Probe Resuspension=== *The 5' amino modified (separated by C6) probes ordered by Dan: **GAD1 ***Already resuspended **FOX...")
 
>Mzcai
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**SATB2
**SATB2
**KIT
**KIT
*Pick 2-3 genes and [[Daniel:Notebook/RNAFISH/2014-8-30#Oligo_Set_Preparations resuspend]] if needed
*Pick 2-3 genes and [[Daniel:Notebook/RNAFISH/2014-8-30#Oligo_Set_Preparations | resuspend]] if needed


===Dye Coupling===
===Dye Coupling===
[[Daniel:Notebook/RNAFACS/2015-4-10#Dye_Labeling Dan's best practice dye coupling protocol]]
[[Daniel:Notebook/RNAFACS/2015-4-10#Dye_Labeling | Dan's best practice dye coupling protocol]]
[[Daniel:Protocols/AlexaDyeCouple#ARES Generic dye coupling protocol]]
[[Daniel:Protocols/AlexaDyeCouple#ARES | Generic dye coupling protocol]]


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===RNA FISH===
===RNA FISH===
[[Daniel:Notebook/RNAFISH/2015-3-4 Dan's best practice FISH protocol]]
[[Daniel:Notebook/RNAFISH/2015-3-4 | Dan's best practice FISH protocol]]
[[Daniel:Protocols/TissueFISH#Linnarsson_Lab_Protocol Linnarsson complete FISH protocol]]
[[Daniel:Protocols/TissueFISH#Linnarsson_Lab_Protocol | Linnarsson complete FISH protocol]]
-->
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Revision as of 02:17, 5 June 2015

RNA FISH: Probe Resuspension and Dye Coupling

Probe Resuspension

  • The 5' amino modified (separated by C6) probes ordered by Dan:
    • GAD1
      • Already resuspended
    • FOXP2
      • Already resuspended
    • CUX2
      • Already resuspended
    • SLC6A1
    • ADARB2
    • SATB2
    • KIT
  • Pick 2-3 genes and resuspend if needed

Dye Coupling

Dan's best practice dye coupling protocol Generic dye coupling protocol