Matt:LabNotes/2015-6-5: Difference between revisions
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>Mzcai (Created page with "==RNA FISH: Probe Resuspension and Dye Coupling== ===Probe Resuspension=== *The 5' amino modified (separated by C6) probes ordered by Dan: **GAD1 ***Already resuspended **FOX...") |
>Mzcai mNo edit summary |
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**SATB2 | **SATB2 | ||
**KIT | **KIT | ||
*Pick 2-3 genes and [[Daniel:Notebook/RNAFISH/2014-8-30#Oligo_Set_Preparations resuspend]] if needed | *Pick 2-3 genes and [[Daniel:Notebook/RNAFISH/2014-8-30#Oligo_Set_Preparations | resuspend]] if needed | ||
===Dye Coupling=== | ===Dye Coupling=== | ||
[[Daniel:Notebook/RNAFACS/2015-4-10#Dye_Labeling Dan's best practice dye coupling protocol]] | [[Daniel:Notebook/RNAFACS/2015-4-10#Dye_Labeling | Dan's best practice dye coupling protocol]] | ||
[[Daniel:Protocols/AlexaDyeCouple#ARES Generic dye coupling protocol]] | [[Daniel:Protocols/AlexaDyeCouple#ARES | Generic dye coupling protocol]] | ||
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===RNA FISH=== | ===RNA FISH=== | ||
[[Daniel:Notebook/RNAFISH/2015-3-4 Dan's best practice FISH protocol]] | [[Daniel:Notebook/RNAFISH/2015-3-4 | Dan's best practice FISH protocol]] | ||
[[Daniel:Protocols/TissueFISH#Linnarsson_Lab_Protocol Linnarsson complete FISH protocol]] | [[Daniel:Protocols/TissueFISH#Linnarsson_Lab_Protocol | Linnarsson complete FISH protocol]] | ||
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Revision as of 02:17, 5 June 2015
RNA FISH: Probe Resuspension and Dye Coupling
Probe Resuspension
- The 5' amino modified (separated by C6) probes ordered by Dan:
- GAD1
- Already resuspended
- FOXP2
- Already resuspended
- CUX2
- Already resuspended
- SLC6A1
- ADARB2
- SATB2
- KIT
- GAD1
- Pick 2-3 genes and resuspend if needed
Dye Coupling
Dan's best practice dye coupling protocol Generic dye coupling protocol