Matt:LabNotes/2015-6-5: Difference between revisions

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===Dye Coupling===
===Dye Coupling===
[[Daniel:Notebook/RNAFACS/2015-4-10#Dye_Labeling | Dan's best practice dye coupling protocol]]
[[Daniel:Notebook/RNAFACS/2015-4-10#Dye_Labeling | Dan's best practice dye coupling protocol]]<br>
[[Daniel:Protocols/AlexaDyeCouple#ARES | Generic dye coupling protocol]]
[[Daniel:Protocols/AlexaDyeCouple#ARES | Generic dye coupling protocol]]



Revision as of 02:18, 5 June 2015

RNA FISH: Probe Resuspension and Dye Coupling

Probe Resuspension

  • The 5' amino modified (separated by C6) probes ordered by Dan:
    • GAD1
      • Already resuspended
    • FOXP2
      • Already resuspended
    • CUX2
      • Already resuspended
    • SLC6A1
    • ADARB2
    • SATB2
    • KIT
  • Pick 2-3 genes and resuspend if needed

Dye Coupling

Dan's best practice dye coupling protocol
Generic dye coupling protocol