Matt:LabNotes/2015-6-5: Difference between revisions
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>Mzcai m (→Dye Coupling) |
>Mzcai m (→Dye Coupling) |
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===Dye Coupling=== | ===Dye Coupling=== | ||
[[Daniel:Notebook/RNAFACS/2015-4-10#Dye_Labeling | Dan's best practice dye coupling protocol]] | [[Daniel:Notebook/RNAFACS/2015-4-10#Dye_Labeling | Dan's best practice dye coupling protocol]]<br> | ||
[[Daniel:Protocols/AlexaDyeCouple#ARES | Generic dye coupling protocol]] | [[Daniel:Protocols/AlexaDyeCouple#ARES | Generic dye coupling protocol]] | ||
Revision as of 02:18, 5 June 2015
RNA FISH: Probe Resuspension and Dye Coupling
Probe Resuspension
- The 5' amino modified (separated by C6) probes ordered by Dan:
- GAD1
- Already resuspended
- FOXP2
- Already resuspended
- CUX2
- Already resuspended
- SLC6A1
- ADARB2
- SATB2
- KIT
- GAD1
- Pick 2-3 genes and resuspend if needed
Dye Coupling
Dan's best practice dye coupling protocol
Generic dye coupling protocol