Matt:LabNotes/2015-7-9: Difference between revisions
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>Mzcai (Created page with "=DARTFISH Imaging Neurons= * Alex's iNGN Bipolar Neurons CA12kNov2014_suppv2 *[[Matt:LabNotes/2015-6-19 | Second Batch of Sebastian's Motor Neuron...") |
>Mzcai |
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*Add 75C pre-heated 0.5uM FISGA_Adpt (Cy3) (1ul 100uM FISGA_Adpt + 199ul 2X SSC + 30% formamide) | *Add 75C pre-heated 0.5uM FISGA_Adpt (Cy3) (1ul 100uM FISGA_Adpt + 199ul 2X SSC + 30% formamide) | ||
*Wash with 2X SSC twice and add 2X SSC for imaging | *Wash with 2X SSC twice and add 2X SSC for imaging | ||
====Alex's Bipolar Neurons==== | |||
*~30 rolonies per cell | |||
[[File:Composite_BipolarNeurons_AdptCy3_Pos1.jpg|450px]] | |||
[[File:Composite_BipolarNeurons_AdptCy3_Pos2.jpg|450px]] | |||
====Sebastian's Motor Neurons==== | |||
*~80 rolonies per cell | |||
[[File:Composite_MotorNeurons_AdptCy3_Pos1.jpg|450px]] | |||
[[File:Composite_MotorNeurons_AdptCy3_Pos2.jpg|450px]] |
Revision as of 02:40, 10 July 2015
DARTFISH Imaging Neurons
- Alex's iNGN Bipolar Neurons CA12kNov2014_suppv2
- Second Batch of Sebastian's Motor Neurons CA12kNov2014_suppv2
Universal Cy3 Dye-Probe check
- Add 75C pre-heated 0.5uM FISGA_Adpt (Cy3) (1ul 100uM FISGA_Adpt + 199ul 2X SSC + 30% formamide)
- Wash with 2X SSC twice and add 2X SSC for imaging
Alex's Bipolar Neurons
- ~30 rolonies per cell
File:Composite BipolarNeurons AdptCy3 Pos1.jpg File:Composite BipolarNeurons AdptCy3 Pos2.jpg
Sebastian's Motor Neurons
- ~80 rolonies per cell
File:Composite MotorNeurons AdptCy3 Pos1.jpg File:Composite MotorNeurons AdptCy3 Pos2.jpg