Matt:LabNotes/2015-7-9: Difference between revisions

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>Mzcai
(Created page with "=DARTFISH Imaging Neurons= * Alex's iNGN Bipolar Neurons CA12kNov2014_suppv2 *[[Matt:LabNotes/2015-6-19 | Second Batch of Sebastian's Motor Neuron...")
 
>Mzcai
 
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*Add 75C pre-heated 0.5uM FISGA_Adpt (Cy3) (1ul 100uM FISGA_Adpt + 199ul 2X SSC + 30% formamide)
*Add 75C pre-heated 0.5uM FISGA_Adpt (Cy3) (1ul 100uM FISGA_Adpt + 199ul 2X SSC + 30% formamide)
*Wash with 2X SSC twice and add 2X SSC for imaging
*Wash with 2X SSC twice and add 2X SSC for imaging
====Alex's Bipolar Neurons====
*~30 rolonies per cell
[[File:Composite_BipolarNeurons_AdptCy3_Pos1.jpg|450px]]
[[File:Composite_BipolarNeurons_AdptCy3_Pos2.jpg|450px]]
====Sebastian's Motor Neurons====
*~80 rolonies per cell
[[File:Composite_MotorNeurons_AdptCy3_Pos1.jpg|450px]]
[[File:Composite_MotorNeurons_AdptCy3_Pos2.jpg|450px]]
====Full Decoding====
*On [[Matt:LabNotes/2015-7-11]]

Latest revision as of 06:45, 13 July 2015

DARTFISH Imaging Neurons[edit]

Universal Cy3 Dye-Probe check[edit]

  • Add 75C pre-heated 0.5uM FISGA_Adpt (Cy3) (1ul 100uM FISGA_Adpt + 199ul 2X SSC + 30% formamide)
  • Wash with 2X SSC twice and add 2X SSC for imaging

Alex's Bipolar Neurons[edit]

  • ~30 rolonies per cell

File:Composite BipolarNeurons AdptCy3 Pos1.jpg File:Composite BipolarNeurons AdptCy3 Pos2.jpg

Sebastian's Motor Neurons[edit]

  • ~80 rolonies per cell

File:Composite MotorNeurons AdptCy3 Pos1.jpg File:Composite MotorNeurons AdptCy3 Pos2.jpg

Full Decoding[edit]