Sam:LabNotes/Microbiome/2009-3-9: Difference between revisions
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>Sam Chiang |
>Sam Chiang |
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==Results== | ==Results== | ||
[[Image:SNAP-151939-0309-sybr-20x-B&W.jpg|SNAP-151939-0309-sybr-20x-B&W]] | [[Image:SNAP-151939-0309-sybr-20x-B&W.jpg|SNAP-151939-0309-sybr-20x-B&W]|600px] | ||
[[Image:SNAP-153406-0004-sybr-20x-fitc.jpg|SNAP-153406-0004-sybr-20x-fitc]] | [[Image:SNAP-153406-0004-sybr-20x-fitc.jpg|SNAP-153406-0004-sybr-20x-fitc|600px]] | ||
[[Image:SNAP-153514-0005-sybr-20x-fitc.jpg|SNAP-153514-0005-sybr-20x-fitc]] | [[Image:SNAP-153514-0005-sybr-20x-fitc.jpg|SNAP-153514-0005-sybr-20x-fitc|600px]] |
Revision as of 23:53, 9 March 2009
E. coli labeling using SYBR Green I and YOYO-1
Objective
- To confirm the bacteria staining with revised procedures
- Using FITC filter, 20X objective, No cover slip
- The stained bacteria will be used for microwell application
Material and Samples
- Samples: Diluted E. coli, 10^6 CFU/uL (stored in PBS, 4C)
- YOYO®-1 iodide (491/509) - 1 mM solution in DMSO
- Diluted SYBR Green I (diluted in H2O, stored in 4C)
Procedures
- Drop 1 uL of E. coli on the glass slide
- Mix the E. coli with either 10 uL 2X SYBR Green I or 2 uL of YOYo-1 dye, gently pipeting for mixing
- Leave the slide in dark box (slide storage box)for incubation 15 min in room temp
Results
[[Image:SNAP-151939-0309-sybr-20x-B&W.jpg|SNAP-151939-0309-sybr-20x-B&W]|600px] SNAP-153406-0004-sybr-20x-fitc SNAP-153514-0005-sybr-20x-fitc