Sam:LabNotes/Microbiome/2009-3-9 exp2: Difference between revisions

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*Based on the previous multiple PCR result, increase the primer concentration for those weak bands
*Based on the previous multiple PCR result, increase the primer concentration for those weak bands
*Test the effect of Q-solution come with Qiagen multiple PCR kit
*Test the effect of (DMSO-like) Q-solution come with Qiagen multiple PCR kit
 


==Material and Samples==
==Material and Samples==

Revision as of 06:36, 10 March 2009

Multiple PCR against human whole genomoe

Objective

  • Based on the previous multiple PCR result, increase the primer concentration for those weak bands
  • Test the effect of (DMSO-like) Q-solution come with Qiagen multiple PCR kit

Material and Samples

  • Prepare the new primer gourp mix for primer set-1(1X = 10 uL, 2X = 20 uL)
    • Set-1 Blue: including Y-1(2X), 21-1(2X), the rest primers (1X)
    • Set-1 Yellow: 11-1(2X), 10-1(2X), 16-1(2X)
    • Set-1 Red: Use the old one
    • Set-1 Green: 18-1(2X), 3-1(2X)
    • Set-1 Orange: 20-1(2X), 7-1(2X)

Procedures