Dinh/Dinh 2015/NOTES/2015-9-16: Difference between revisions

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>Dinh
(Created page with "= RRBS with input 1.5 ng from plasma DNA = {| class="wikitable" | align="center" style="background:#f0f0f0;"|'''Plasma Sample''' | align="center" style="background:#f0f0f0;"|'...")
 
>Dinh
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= RRBS with input 1.5 ng from plasma DNA =
= RRBS with input 1.5 ng from plasma DNA =
* There are a total of 40 samples to be processed.
* TruSeq adaptors have a total of 24 different barcodes.
{| class="wikitable"
{| class="wikitable"
| align="center" style="background:#f0f0f0;"|'''Plasma Sample'''
| align="center" style="background:#f0f0f0;"|'''Plasma Sample'''

Revision as of 18:49, 17 September 2015

RRBS with input 1.5 ng from plasma DNA

  • There are a total of 40 samples to be processed.
  • TruSeq adaptors have a total of 24 different barcodes.
Plasma Sample Sample Concentration (ng/uL) Volume eluted in EB (uL) Yield (ng) Plasma volume (uL) Est. yield from 1 mL (ng) Volume for 1.5 ng (uL) Volume water to make 12 uL (uL)
7.12 0.16 30 4.68 535 8.75 9.62 2.38
6.27 0.17 30 5.10 55 92.73 8.82 3.18
6.20 0.18 30 5.40 641 8.42 8.33 3.67
6.15 0.19 30 5.82 610 9.54 7.73 4.27
6.25 0.23 30 6.96 150 46.40 6.47 5.53
6.11 0.25 30 7.38 231 31.95 6.10 5.90
7.26 0.26 30 7.92 690 11.48 5.68 6.32
6.17 0.27 30 7.98 480 16.63 5.64 6.36
7.29 0.28 30 8.28 690 12.00 5.43 6.57
7.25 0.29 30 8.76 700 12.51 5.14 6.86
7.16 0.29 30 8.82 630 14.00 5.10 6.90
6.12 0.31 30 9.36 670 13.97 4.81 7.19
6.29 0.31 30 9.42 940 10.02 4.78 7.22
6.18 0.32 30 9.48 425 22.31 4.75 7.25
7.13 0.32 30 9.66 630 15.33 4.66 7.34
7.15 0.32 30 9.66 455 21.23 4.66 7.34
6.26 0.33 30 9.84 550 17.89 4.57 7.43
6.24 0.37 30 10.98 725 15.14 4.10 7.90
7.21 0.37 30 11.04 620 17.81 4.08 7.92
7.14 0.37 30 11.10 600 18.50 4.05 7.95
6.21 0.38 30 11.40 670 17.01 3.95 8.05
6.16 0.39 30 11.76 650 18.09 3.83 8.17
6.19 0.39 30 11.82 650 18.18 3.81 8.19
7.11 0.42 30 12.66 595 21.28 3.55 8.45
6.30 0.43 30 12.84 405 31.70 3.50 8.50
7.19 0.44 30 13.20 760 17.37 3.41 8.59
6.14 0.48 30 14.28 585 24.41 3.15 8.85
7.27 0.55 30 16.50 700 23.57 2.73 9.27
7.20 0.75 30 22.38 550 40.69 2.01 9.99
7.22 0.77 30 23.04 385 59.84 1.95 10.05
6.23 0.88 30 26.34 900 29.27 1.71 10.29
ALL 0.94 30 28.08 NA NA 1.60 10.40
7.18 0.71 40 28.24 430 65.67 2.12 9.88
6.13 1.04 30 31.26 690 45.30 1.44 10.56
7.28 1.06 30 31.80 780 40.77 1.42 10.58
7.17 1.09 30 32.58 355 91.77 1.38 10.62
6.22 1.29 30 38.64 460 84.00 1.16 10.84
7.23 1.20 40 48.00 540 88.89 1.25 10.75
7.30 2.32 30 69.60 398 174.87 0.65 11.35
6.28 4.08 30 122.40 940 130.21 0.37 11.63
7.24 106.00 40 4240.00 685 6189.78 0.01 11.99