Daniel:Notebook/RNAFACS/2015-10-19: Difference between revisions

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>Djacobse
(Created page with "=RNA Scope Trial Run 3 (Pre-Sorted Nuclei; Started 10/17/2015))= Back to Calendar ==Microscopy Results== ===Positive Control=== ...")
 
>Djacobse
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===Positive Control===
===Positive Control===
No images uploaded.  I couldn't find much on this one, although that could be because I couldn't find any nuclei.  Re-examination might show some, but given the results from experiment I'm not sure it would be very helpful.


===Experiment===
===Experiment===
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File:20151017_Experiment.lif_Series004Snapshot_All1_ch00.jpg|Field 5
File:20151017_Experiment.lif_Series004Snapshot_All1_ch00.jpg|Field 5
</gallery>
</gallery>
It is harder to see on this computer, but looking at the images closely, you can see distinct overlap in the yellow (RBFOX3/NeuN) and the red (DRAQ5) channels.  I think this was a successful trial run.

Revision as of 16:34, 19 October 2015

RNA Scope Trial Run 3 (Pre-Sorted Nuclei; Started 10/17/2015))

Back to Calendar

Microscopy Results

Positive Control

No images uploaded. I couldn't find much on this one, although that could be because I couldn't find any nuclei. Re-examination might show some, but given the results from experiment I'm not sure it would be very helpful.

Experiment

DRAQ-5 in Red; RBFOX3 (NeuN) in yellow; FEZF2 in green

It is harder to see on this computer, but looking at the images closely, you can see distinct overlap in the yellow (RBFOX3/NeuN) and the red (DRAQ5) channels. I think this was a successful trial run.