Daniel:Notebook/RNAFACS/2015-10-19: Difference between revisions

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>Djacobse
(Created page with "=RNA Scope Trial Run 3 (Pre-Sorted Nuclei; Started 10/17/2015))= Back to Calendar ==Microscopy Results== ===Positive Control=== ...")
 
>Djacobse
 
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=RNA Scope Trial Run 3 (Pre-Sorted Nuclei; Started [[2015-10-17|10/17/2015]]))=
=RNA Scope Trial Run 3 (Pre-Sorted Nuclei; Started [[Daniel:Notebook/RNAFACS/2015-10-17|10/17/2015]]))=


[[Daniel:Notebook/RNAFACS|Back to Calendar]]
[[Daniel:Notebook/RNAFACS|Back to Calendar]]
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===Positive Control===
===Positive Control===
No images uploaded.  I couldn't find much on this one, although that could be because I couldn't find any nuclei.  Re-examination might show some, but given the results from experiment I'm not sure it would be very helpful.


===Experiment===
===Experiment===
'''DRAQ-5 in Red; RBFOX3 (NeuN) in yellow; FEZF2 in green'''
'''DRAQ-5 in Red; RBFOX3 (NeuN) in yellow; FEZF2 in green'''
Images taken using max projection, 63X lens, and 2.5X zoom.  Approximate pixel size = 72nm x 72nm. Total field of view: 73 um x 73 um


<gallery>
<gallery widths=300px heights=300px perrow=2>
File:20151017_Experiment.lif_Series018Snapshot_All1_ch00.jpg|Field 1
File:20151017_Experiment.lif_Series018Snapshot_All1_ch00.jpg|Field 1
File:20151017_Experiment.lif_Series002Snapshot_All1_ch00.jpg|Field 2
File:20151017_Experiment.lif_Series002Snapshot_All1_ch00.jpg|Field 2
Line 17: Line 20:
File:20151017_Experiment.lif_Series004Snapshot_All1_ch00.jpg|Field 5
File:20151017_Experiment.lif_Series004Snapshot_All1_ch00.jpg|Field 5
</gallery>
</gallery>
It is harder to see on this computer, but looking at the images closely, you can see distinct overlap in the yellow (RBFOX3/NeuN) and the red (DRAQ5) channels.  I think this was a successful trial run.

Latest revision as of 18:49, 19 October 2015

RNA Scope Trial Run 3 (Pre-Sorted Nuclei; Started 10/17/2015))[edit]

Back to Calendar

Microscopy Results[edit]

Positive Control[edit]

No images uploaded. I couldn't find much on this one, although that could be because I couldn't find any nuclei. Re-examination might show some, but given the results from experiment I'm not sure it would be very helpful.

Experiment[edit]

DRAQ-5 in Red; RBFOX3 (NeuN) in yellow; FEZF2 in green Images taken using max projection, 63X lens, and 2.5X zoom. Approximate pixel size = 72nm x 72nm. Total field of view: 73 um x 73 um

It is harder to see on this computer, but looking at the images closely, you can see distinct overlap in the yellow (RBFOX3/NeuN) and the red (DRAQ5) channels. I think this was a successful trial run.