Daniel:Notebook/RNAFACS/2015-10-19: Difference between revisions
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>Djacobse (Created page with "=RNA Scope Trial Run 3 (Pre-Sorted Nuclei; Started 10/17/2015))= Back to Calendar ==Microscopy Results== ===Positive Control=== ...") |
>Djacobse |
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=RNA Scope Trial Run 3 (Pre-Sorted Nuclei; Started [[2015-10-17|10/17/2015]]))= | =RNA Scope Trial Run 3 (Pre-Sorted Nuclei; Started [[Daniel:Notebook/RNAFACS/2015-10-17|10/17/2015]]))= | ||
[[Daniel:Notebook/RNAFACS|Back to Calendar]] | [[Daniel:Notebook/RNAFACS|Back to Calendar]] | ||
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===Positive Control=== | ===Positive Control=== | ||
No images uploaded. I couldn't find much on this one, although that could be because I couldn't find any nuclei. Re-examination might show some, but given the results from experiment I'm not sure it would be very helpful. | |||
===Experiment=== | ===Experiment=== | ||
'''DRAQ-5 in Red; RBFOX3 (NeuN) in yellow; FEZF2 in green''' | '''DRAQ-5 in Red; RBFOX3 (NeuN) in yellow; FEZF2 in green''' | ||
Images taken using max projection, 63X lens, and 2.5X zoom. Approximate pixel size = 72nm x 72nm. Total field of view: 73 um x 73 um | |||
<gallery> | <gallery widths=300px heights=300px perrow=2> | ||
File:20151017_Experiment.lif_Series018Snapshot_All1_ch00.jpg|Field 1 | File:20151017_Experiment.lif_Series018Snapshot_All1_ch00.jpg|Field 1 | ||
File:20151017_Experiment.lif_Series002Snapshot_All1_ch00.jpg|Field 2 | File:20151017_Experiment.lif_Series002Snapshot_All1_ch00.jpg|Field 2 | ||
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File:20151017_Experiment.lif_Series004Snapshot_All1_ch00.jpg|Field 5 | File:20151017_Experiment.lif_Series004Snapshot_All1_ch00.jpg|Field 5 | ||
</gallery> | </gallery> | ||
It is harder to see on this computer, but looking at the images closely, you can see distinct overlap in the yellow (RBFOX3/NeuN) and the red (DRAQ5) channels. I think this was a successful trial run. |
Latest revision as of 18:49, 19 October 2015
RNA Scope Trial Run 3 (Pre-Sorted Nuclei; Started 10/17/2015))[edit]
Microscopy Results[edit]
Positive Control[edit]
No images uploaded. I couldn't find much on this one, although that could be because I couldn't find any nuclei. Re-examination might show some, but given the results from experiment I'm not sure it would be very helpful.
Experiment[edit]
DRAQ-5 in Red; RBFOX3 (NeuN) in yellow; FEZF2 in green Images taken using max projection, 63X lens, and 2.5X zoom. Approximate pixel size = 72nm x 72nm. Total field of view: 73 um x 73 um
- 20151017 Experiment.lif Series018Snapshot All1 ch00.jpg
Field 1
- 20151017 Experiment.lif Series002Snapshot All1 ch00.jpg
Field 2
- 20151017 Experiment.lif Series006Snapshot All1 ch00.jpg
Field 3
- 20151017 Experiment.lif Series008Snapshot All1 ch00.jpg
Field 4
- 20151017 Experiment.lif Series004Snapshot All1 ch00.jpg
Field 5
It is harder to see on this computer, but looking at the images closely, you can see distinct overlap in the yellow (RBFOX3/NeuN) and the red (DRAQ5) channels. I think this was a successful trial run.