Daniel:Notebook/RNAFACS/2015-10-28: Difference between revisions

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>Djacobse
>Djacobse
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File:20151028_PositiveControl.lif_Series011Snapshot1_ch00.jpg|Field 4
File:20151028_PositiveControl.lif_Series011Snapshot1_ch00.jpg|Field 4
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It's possible that these are signal, but I can't tell because they overlap.  I checked with the positive controls (POLR2A, PPIB, UBC) and they are present (according to gene cards), but I can't tell if RNA should be in the nucleus or not.  Technically, most genes being transcribed probably are, but they need to be present in the nucleus for this to work.


===Experiment (20X)===
===Experiment (20X)===
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File:20151028_Experiment.lif_Series007Snapshot1_ch00.jpg|Field 3
File:20151028_Experiment.lif_Series007Snapshot1_ch00.jpg|Field 3
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===Experiment (10X)===
===Experiment (10X)===

Revision as of 23:38, 28 October 2015

RNA Scope Trial Run 5: Pretreat III

Back to Calender

Microscopy

Positive Control (20X)

It's possible that these are signal, but I can't tell because they overlap. I checked with the positive controls (POLR2A, PPIB, UBC) and they are present (according to gene cards), but I can't tell if RNA should be in the nucleus or not. Technically, most genes being transcribed probably are, but they need to be present in the nucleus for this to work.

Experiment (20X)


Experiment (10X)