Daniel:Notebook/RNAFACS/2015-10-30: Difference between revisions

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>Djacobse
(Created page with "=RNA Scope Trial Run 5: 10X-I= Back to Calender ==Microcscopy Results== So I tried to look at the results under the 10X. Unfortunately, I check...")
 
>Djacobse
 
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==Microcscopy Results==
==Microcscopy Results==


So I tried to look at the results under the 10X.  Unfortunately, I check and DAPI quenches DRAQ5. There is a lot of signal under the DAPI channel, but I cannot visualize the nuclei, which makes it very difficult to take images.
So I tried to look at the results under the 10X.  Unfortunately, I [http://www.abcam.com/ps/products/108/ab108410/documents/ab108410%20DRAQ5%20v2%20(Web).pdf checked] and DAPI quenches DRAQ5. There is a lot of signal under the DAPI channel, but I cannot visualize the nuclei, which makes it very difficult to take images.


<gallery heights=300px widths=300px perrow=3 caption="Taken with 63X oil on confocal; max z-plane; Red=Draq5 (C2), Green=FEZF2 (C3); Yellow=RBFOX3/NeuN (C1)">
File:20151030.lif_Series001Snapshot1_ch00.jpg|Field 1
File:20151030.lif_Series003Snapshot1_ch00.jpg|Field 2
File:20151030.lif_Series004Snapshot1_ch00.jpg|Field 3; several nuclei in this frame, only 1 shows both FEZF2 and RBFOX3 signal
</gallery>


[[Category:RNAFACS]] [[Category:RNAScope]] [[Category:RNAScope-10X-I]]
[[Category:RNAFACS]] [[Category:RNAScope]] [[Category:RNAScope-10X-I]]

Latest revision as of 16:12, 30 October 2015

RNA Scope Trial Run 5: 10X-I[edit]

Back to Calender

Microcscopy Results[edit]

So I tried to look at the results under the 10X. Unfortunately, I checked and DAPI quenches DRAQ5. There is a lot of signal under the DAPI channel, but I cannot visualize the nuclei, which makes it very difficult to take images.