Daniel:Notebook/RNAFACS/2015-12-1: Difference between revisions
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>Djacobse |
>Djacobse |
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[[Daniel:Notebook/RNAFACS|Back to Calendar]] | [[Daniel:Notebook/RNAFACS|Back to Calendar]] | ||
Didn't have time today to start testing, so I'll do it [[Daniel:Notebook/RNAFACS/2015-12-2|tomorrow]]. Today I prepped fresh ALS and NS and tested against each other. From now on, I'll use the below as my formula. | |||
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===Buffers=== | ===Buffers=== | ||
*Alkaline Lysis Solution (ALS) | *Alkaline Lysis Solution (ALS) | ||
Line 91: | Line 13: | ||
**2 ul 0.5 M EDTA | **2 ul 0.5 M EDTA | ||
*Neutralization Solution (NS) | *Neutralization Solution (NS) | ||
** | **65 uL H20 | ||
** | **15 uL 4M Tris-HCl | ||
** | **20 uL 2N HCl |
Latest revision as of 16:53, 2 December 2015
PA Gel Slide Testing[edit]
Didn't have time today to start testing, so I'll do it tomorrow. Today I prepped fresh ALS and NS and tested against each other. From now on, I'll use the below as my formula.
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Buffers[edit]
- Alkaline Lysis Solution (ALS)
- 80 ul Nuclease free H2O.
- 8 ul 5 M KOH.
- 10 ul 1 M DTT.
- 2 ul 0.5 M EDTA
- Neutralization Solution (NS)
- 65 uL H20
- 15 uL 4M Tris-HCl
- 20 uL 2N HCl