Ns126:Calendar/NOTES/2016-1-5: Difference between revisions
Jump to navigation
Jump to search
>Shicheng |
>Shicheng |
||
Line 31: | Line 31: | ||
--comprehensive \ | --comprehensive \ | ||
--output ../methyfreq \ | --output ../methyfreq \ | ||
.bam | ENCFF000LUN.fastq_qual_trimmed.fastq.bam | ||
===Methylation Haplotype Load Matrix=== | ===Methylation Haplotype Load Matrix=== |
Revision as of 09:30, 7 January 2016
Collection RRBS Dataset From Richard Myers, HAIB (Encode Project)
Data Description
Fastq Download
- Fastq donwload address: File:Haib.download.files.txt
xargs -n 1 curl -O -L < haib.download.files.txt
Check Phred Score
perl ~/bin/checkphred.pl *fastq
Fastq Quality Control
trim_galore --phred64 --fastqc --illumina --non_directional --rrbs *.fastq
Fastq Alignment
bismark --bowtie2 --phred64-quals --fastq -L 30 -N 1 /home/shg047/db/aligndb/hg19/bismark -1 ENCFF000LUN.fastq_qual_trimmed.fastq -o ../bam
Methylation haploinfo
bismark_methylation_extractor \ --single-end \ --bedGraph \ --buffer_size 2G \ --remove_spaces \ --zero_based \ --merge_non_CpG \ --comprehensive \ --output ../methyfreq \ ENCFF000LUN.fastq_qual_trimmed.fastq.bam