Daniel:Notebook/ComboLock/CProbeDesign: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
(Created page with "=C Probe Design= Back to Notebook This page describes the methods used for designing C-Probes for Combo Lock. The illustration below shows wha...")
 
>Djacobse
Line 6: Line 6:


[[Image:C_Probes.png|600px]]
[[Image:C_Probes.png|600px]]
==Designing Transcript Matching Region==
*Taken from [http://www.nature.com/nmeth/journal/v13/n3/abs/nmeth.3742.html Frei et al paper]
*[https://github.com/nolanlab/PLAYRDesign/blob/master/README.md README] for probe design software
#Must install R, Primer3, and Blast+ before using
#Make blast data
#In R:
library(devtools)
install_github("nolanlab/PLAYRDesign")
library(PLAYRDesign)

Revision as of 16:08, 21 April 2016

C Probe Design

Back to Notebook

This page describes the methods used for designing C-Probes for Combo Lock. The illustration below shows what a C-probe is expected to look like. The descriptions given include the original design sizes.

File:C Probes.png

Designing Transcript Matching Region

  1. Must install R, Primer3, and Blast+ before using
  2. Make blast data
  3. In R:
library(devtools)
install_github("nolanlab/PLAYRDesign")
library(PLAYRDesign)