Daniel:Notebook/ComboLock/2016-6-29: Difference between revisions

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>Djacobse
(Created page with "=Antibody Stage 2 Test 2 (Started yesterday)= Back to Calendar ==Protcol== <ol start="2"> <li>Latch an...")
 
>Djacobse
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<li>Exonuclease Digestion</li>
<li>Exonuclease Digestion</li>
<ol type="A">
<ol type="A">
<li>Prepare exonuclease I/III mix by mixing 2 uL exonuclease I (20 units/μL) and 10 uL exonuclease III (100 units/μL) in 1:1 ratio</li>
<li>Prepare exonuclease I/III mix by mixing 15 uL exonuclease I (20 units/μL) and 3 uL exonuclease III (100 units/μL) in 1:1 ratio</li>
<li>Add 2 μL of exonuclease I/III mix to reaction</li>
<li>Add 2 μL of exonuclease I/III mix to reaction</li>
<li>Mix the reaction by swirling pipette around the well 5 times</li>
<li>Mix the reaction by swirling pipette around the well 5 times</li>

Revision as of 18:31, 29 June 2016

Antibody Stage 2 Test 2 (Started yesterday)

Back to Calendar

Protcol

  1. Latch and Padlock Hybridization
    1. Heat inactivate enzyme by incubating for 2 minutes at 94C
  2. Exonuclease Digestion
    1. Prepare exonuclease I/III mix by mixing 15 uL exonuclease I (20 units/μL) and 3 uL exonuclease III (100 units/μL) in 1:1 ratio
    2. Add 2 μL of exonuclease I/III mix to reaction
    3. Mix the reaction by swirling pipette around the well 5 times
    4. Incubate reaction at 37 ºC for 2 hours
    5. Heat inactivate enzyme by incubating at 94C for 5 minutes