Daniel:Notebook/ComboLock/2016-6-29: Difference between revisions
Jump to navigation
Jump to search
>Djacobse (Created page with "=Antibody Stage 2 Test 2 (Started yesterday)= Back to Calendar ==Protcol== <ol start="2"> <li>Latch an...") |
>Djacobse |
||
Line 12: | Line 12: | ||
<li>Exonuclease Digestion</li> | <li>Exonuclease Digestion</li> | ||
<ol type="A"> | <ol type="A"> | ||
<li>Prepare exonuclease I/III mix by mixing | <li>Prepare exonuclease I/III mix by mixing 15 uL exonuclease I (20 units/μL) and 3 uL exonuclease III (100 units/μL) in 1:1 ratio</li> | ||
<li>Add 2 μL of exonuclease I/III mix to reaction</li> | <li>Add 2 μL of exonuclease I/III mix to reaction</li> | ||
<li>Mix the reaction by swirling pipette around the well 5 times</li> | <li>Mix the reaction by swirling pipette around the well 5 times</li> |
Revision as of 18:31, 29 June 2016
Antibody Stage 2 Test 2 (Started yesterday)
Protcol
- Latch and Padlock Hybridization
- Heat inactivate enzyme by incubating for 2 minutes at 94C
- Exonuclease Digestion
- Prepare exonuclease I/III mix by mixing 15 uL exonuclease I (20 units/μL) and 3 uL exonuclease III (100 units/μL) in 1:1 ratio
- Add 2 μL of exonuclease I/III mix to reaction
- Mix the reaction by swirling pipette around the well 5 times
- Incubate reaction at 37 ºC for 2 hours
- Heat inactivate enzyme by incubating at 94C for 5 minutes