Daniel:Notebook/ComboLock/2016-7-7: Difference between revisions

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(Created page with "=Sequential Hybridization Test (Started 7-5-2016)= Back to Calendar ==Protocol== <ol start="6"> <li>Eth...")
 
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{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="background-color:#DDD9C4;font-size:12pt;font-weight:bold" align="center"
|- style="background-color:#DDD9C4;font-size:12pt;font-weight:bold" align="center"
| width="80" height="15" |  Source
| width="105" height="15" |  Source
| width="65" | ng/uL
| width="65" | ng/uL



Revision as of 16:03, 7 July 2016

Sequential Hybridization Test (Started 7-5-2016)

Back to Calendar

Protocol

  1. Ethanol precipitation (from yesterday)
    1. Centrifuge at 4 C for 25 minutes at 14000 rpm
    2. Remove supernatant and add 750 uL chilled 75% EtOH
    3. Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
    4. Remove supernatant and dry the pellet in the hood
    5. Resuspend pellet in 20 uL volume nfH20
    6. Measure in Nanodrop
    7. Store DNA at 4C

Nanodrop Results

Source ng/uL
C Probe- Seq Tube 1  
C Probe-Seq Tube 2