Daniel:Notebook/ComboLock/2016-7-7: Difference between revisions
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>Djacobse (Created page with "=Sequential Hybridization Test (Started 7-5-2016)= Back to Calendar ==Protocol== <ol start="6"> <li>Eth...") |
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| width="65" | ng/uL | | width="65" | ng/uL | ||
Revision as of 16:03, 7 July 2016
Sequential Hybridization Test (Started 7-5-2016)
Protocol
- Ethanol precipitation (from yesterday)
- Centrifuge at 4 C for 25 minutes at 14000 rpm
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
- Remove supernatant and dry the pellet in the hood
- Resuspend pellet in 20 uL volume nfH20
- Measure in Nanodrop
- Store DNA at 4C
Nanodrop Results
Source | ng/uL |
C Probe- Seq Tube 1 | |
C Probe-Seq Tube 2 |