Daniel:Notebook/ComboLock/2016-7-7: Difference between revisions
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==Library Prep== | |||
Mixed samples according to table. | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="background-color:#DDD9C4;font-size:12pt;font-weight:bold" align="center" | |||
| width="65" height="30" | Sample | |||
| width="65" | ng/uL | |||
| width="65" | uL added | |||
| width="65" | final mass (ug) | |||
|- style="font-size:12pt" | |||
| height="30" | C Probe-Seq Tube 1 | |||
| align="center" align="center" | 243.1 | |||
| align="center" align="center" | 15 | |||
| align="center" align="center" | 3.6465 | |||
|- style="background-color:#D9D9D9;font-size:12pt" | |||
| height="30" | C Probe-Seq Tube 2 | |||
| align="center" align="center" | 287.9 | |||
| align="center" align="center" | 15 | |||
| align="center" align="center" | 4.3185 | |||
|} | |||
Sample Name: '''DEJ-CL-C03''' | |||
[[Category:ComboLock]] [[Category:20160705]] | [[Category:ComboLock]] [[Category:20160705]] |
Revision as of 17:20, 7 July 2016
Sequential Hybridization Test (Started 7-5-2016)
Protocol
- Ethanol precipitation (from yesterday)
- Centrifuge at 4 C for 25 minutes at 14000 rpm
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
- Remove supernatant and dry the pellet in the hood
- Resuspend pellet in 20 uL volume nfH20
- Measure in Nanodrop
- Store DNA at 4C
Nanodrop Results
Source | ng/uL |
C Probe-Seq Tube 1 | 243.1 |
C Probe-Seq Tube 2 | 287.9 |
Library Prep
Mixed samples according to table.
Sample | ng/uL | uL added | final mass (ug) |
C Probe-Seq Tube 1 | 243.1 | 15 | 3.6465 |
C Probe-Seq Tube 2 | 287.9 | 15 | 4.3185 |
Sample Name: DEJ-CL-C03