Daniel:Notebook/ComboLock/2016-7-7: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
>Djacobse
Line 49: Line 49:
| align="center" align="center" | 243.1
| align="center" align="center" | 243.1
| align="center" align="center" | 15
| align="center" align="center" | 15
| align="center" align="center" | 3.6465
| align="center" align="center" | 3.6


|- style="background-color:#D9D9D9;font-size:12pt"
|- style="background-color:#D9D9D9;font-size:12pt"
Line 55: Line 55:
| align="center" align="center" | 287.9
| align="center" align="center" | 287.9
| align="center" align="center" | 15
| align="center" align="center" | 15
| align="center" align="center" | 4.3185
| align="center" align="center" | 4.3


|}
|}

Revision as of 17:30, 7 July 2016

Sequential Hybridization Test (Started 7-5-2016)

Back to Calendar

Protocol

  1. Ethanol precipitation (from yesterday)
    1. Centrifuge at 4 C for 25 minutes at 14000 rpm
    2. Remove supernatant and add 750 uL chilled 75% EtOH
    3. Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
    4. Remove supernatant and dry the pellet in the hood
    5. Resuspend pellet in 20 uL volume nfH20
    6. Measure in Nanodrop
    7. Store DNA at 4C

Nanodrop Results

Source ng/uL
C Probe-Seq Tube 1 243.1
C Probe-Seq Tube 2 287.9

Library Prep

Mixed samples according to table.

Sample ng/uL uL added final mass (ug)
C Probe-Seq Tube 1 243.1 15 3.6
C Probe-Seq Tube 2 287.9 15 4.3

Sample Name: DEJ-CL-C03