Daniel:Notebook/ComboLock/2016-7-7: Difference between revisions
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| align="center" align="center" | 243.1 | | align="center" align="center" | 243.1 | ||
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| align="center" align="center" | 3. | | align="center" align="center" | 3.6 | ||
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| align="center" align="center" | 287.9 | | align="center" align="center" | 287.9 | ||
| align="center" align="center" | 15 | | align="center" align="center" | 15 | ||
| align="center" align="center" | 4. | | align="center" align="center" | 4.3 | ||
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Revision as of 17:30, 7 July 2016
Sequential Hybridization Test (Started 7-5-2016)
Protocol
- Ethanol precipitation (from yesterday)
- Centrifuge at 4 C for 25 minutes at 14000 rpm
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
- Remove supernatant and dry the pellet in the hood
- Resuspend pellet in 20 uL volume nfH20
- Measure in Nanodrop
- Store DNA at 4C
Nanodrop Results
Source | ng/uL |
C Probe-Seq Tube 1 | 243.1 |
C Probe-Seq Tube 2 | 287.9 |
Library Prep
Mixed samples according to table.
Sample | ng/uL | uL added | final mass (ug) |
C Probe-Seq Tube 1 | 243.1 | 15 | 3.6 |
C Probe-Seq Tube 2 | 287.9 | 15 | 4.3 |
Sample Name: DEJ-CL-C03