Daniel:Notebook/ComboLock/2016-7-13: Difference between revisions
Jump to navigation
Jump to search
>Djacobse |
>Djacobse |
||
Line 21: | Line 21: | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | ||
|- style="background-color:#DDD9C4;font-size:12pt;font-weight:bold" align="center" | |- style="background-color:#DDD9C4;font-size:12pt;font-weight:bold" align="center" | ||
| width=" | | width="105" height="15" | Source | ||
| width="65" | ng/uL | | width="65" | ng/uL | ||
Latest revision as of 21:44, 13 July 2016
Million Fold Test (Started 7-11-16)[edit]
Protocol[edit]
- Ethanol precipitation (from yesterday)
- Centrifuge at 4 C for 25 minutes at 14000 rpm
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
- Remove supernatant and dry the pellet in the hood
- Resuspend pellet in 20 uL volume nfH20
- Measure in Nanodrop
- Store DNA at 4C
Nanodrop Results[edit]
Source | ng/uL |
Million Fold-Tube A | 37.1 |
Million Fold-Tube B | 30.1 |